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Lactobacillus reuteri DSM 20016 produces cobalamin-dependent diol dehydratase in metabolosomes and metabolizes 1,2-propanediol by disproportionation.

Publication ,  Journal Article
Sriramulu, DD; Liang, M; Hernandez-Romero, D; Raux-Deery, E; Lünsdorf, H; Parsons, JB; Warren, MJ; Prentice, MB
Published in: Journal of bacteriology
July 2008

A Lactobacillus reuteri strain isolated from sourdough is known to produce the vitamin cobalamin. The organism requires this for glycerol cofermentation by a cobalamin-dependent enzyme, usually termed glycerol dehydratase, in the synthesis of the antimicrobial substance reuterin. We show that the cobalamin-synthesizing capacity of another L. reuteri strain (20016, the type strain, isolated from the human gut and recently sequenced as F275) is genetically and phenotypically linked, as in the Enterobacteriaceae, to the production of a cobalamin-dependent enzyme which is associated with a bacterial microcompartment (metabolosome) and known as diol dehydratase. We show that this enzyme allows L. reuteri to carry out a disproportionation reaction converting 1,2-propanediol to propionate and propanol. The wide distribution of this operon suggests that it is adapted to horizontal transmission between bacteria. However, there are significant genetic and phenotypic differences between the Lactobacillus background and the Enterobacteriaceae. Electron microscopy reveals that the bacterial microcompartment in L. reuteri occupies a smaller percentage of the cytoplasm than in gram-negative bacteria. DNA sequence data show evidence of a regulatory control mechanism different from that in gram-negative bacteria, with the presence of a catabolite-responsive element (CRE) sequence immediately upstream of the pdu operon encoding diol dehydratase and metabolosome structural genes in L. reuteri. The metabolosome-associated diol dehydratase we describe is the only candidate glycerol dehydratase present on inspection of the L. reuteri F275 genome sequence.

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Published In

Journal of bacteriology

DOI

EISSN

1098-5530

ISSN

0021-9193

Publication Date

July 2008

Volume

190

Issue

13

Start / End Page

4559 / 4567

Related Subject Headings

  • Vitamin B 12
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
  • Sequence Analysis, DNA
  • Propylene Glycol
  • Propionates
  • Propanediol Dehydratase
  • Propane
  • Polymerase Chain Reaction
  • Operon
  • Molecular Sequence Data
 

Citation

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Sriramulu, D. D., Liang, M., Hernandez-Romero, D., Raux-Deery, E., Lünsdorf, H., Parsons, J. B., … Prentice, M. B. (2008). Lactobacillus reuteri DSM 20016 produces cobalamin-dependent diol dehydratase in metabolosomes and metabolizes 1,2-propanediol by disproportionation. Journal of Bacteriology, 190(13), 4559–4567. https://doi.org/10.1128/jb.01535-07
Sriramulu, Dinesh Diraviam, Mingzhi Liang, Diana Hernandez-Romero, Evelyne Raux-Deery, Heinrich Lünsdorf, Joshua B. Parsons, Martin J. Warren, and Michael B. Prentice. “Lactobacillus reuteri DSM 20016 produces cobalamin-dependent diol dehydratase in metabolosomes and metabolizes 1,2-propanediol by disproportionation.Journal of Bacteriology 190, no. 13 (July 2008): 4559–67. https://doi.org/10.1128/jb.01535-07.
Sriramulu DD, Liang M, Hernandez-Romero D, Raux-Deery E, Lünsdorf H, Parsons JB, et al. Lactobacillus reuteri DSM 20016 produces cobalamin-dependent diol dehydratase in metabolosomes and metabolizes 1,2-propanediol by disproportionation. Journal of bacteriology. 2008 Jul;190(13):4559–67.
Sriramulu, Dinesh Diraviam, et al. “Lactobacillus reuteri DSM 20016 produces cobalamin-dependent diol dehydratase in metabolosomes and metabolizes 1,2-propanediol by disproportionation.Journal of Bacteriology, vol. 190, no. 13, July 2008, pp. 4559–67. Epmc, doi:10.1128/jb.01535-07.
Sriramulu DD, Liang M, Hernandez-Romero D, Raux-Deery E, Lünsdorf H, Parsons JB, Warren MJ, Prentice MB. Lactobacillus reuteri DSM 20016 produces cobalamin-dependent diol dehydratase in metabolosomes and metabolizes 1,2-propanediol by disproportionation. Journal of bacteriology. 2008 Jul;190(13):4559–4567.

Published In

Journal of bacteriology

DOI

EISSN

1098-5530

ISSN

0021-9193

Publication Date

July 2008

Volume

190

Issue

13

Start / End Page

4559 / 4567

Related Subject Headings

  • Vitamin B 12
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
  • Sequence Analysis, DNA
  • Propylene Glycol
  • Propionates
  • Propanediol Dehydratase
  • Propane
  • Polymerase Chain Reaction
  • Operon
  • Molecular Sequence Data