Loss-of-Function RNAi Screen to Identify Necrosis-Signaling Molecules.

Journal Article

Over the recent years, genome-wide RNA interference (RNAi) library screens have been instrumental in the identification of key regulators of various biological pathways. The prolific use of this technique is attributed to its amenability to a high-throughput format. Here, we present the step-by-step method to conduct a siRNA screen to identify genes involved in necroptosis, a nonapoptotic form of proinflammatory cell death. The method described here uses MTS cell proliferation assay to measure necroptosis, which is compatible with high-throughput format screening on multiwell microtiter plates. This ensures that the screen can be performed in a timely and efficient manner.

Full Text

Duke Authors

Cited Authors

  • Moquin, DM; Chan, FK-M

Published Date

  • January 2018

Published In

Volume / Issue

  • 1857 /

Start / End Page

  • 11 - 18

PubMed ID

  • 30136226

Pubmed Central ID

  • 30136226

Electronic International Standard Serial Number (EISSN)

  • 1940-6029

International Standard Serial Number (ISSN)

  • 1064-3745

Digital Object Identifier (DOI)

  • 10.1007/978-1-4939-8754-2_2


  • eng