Rapid Antimicrobial Susceptibility Testing Using Forward Laser Light Scatter Technology.


Journal Article

The delayed reporting of antimicrobial susceptibility testing remains a limiting factor in clinical decision-making in the treatment of bacterial infection. This study evaluates the use of forward laser light scatter (FLLS) to measure bacterial growth for the early determination of antimicrobial susceptibility. Three isolates each (two clinical isolates and one reference strain) of Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa were tested in triplicate using two commercial antimicrobial testing systems, the Vitek2 and the MicroScan MIC panel, to challenge the BacterioScan FLLS. The BacterioScan FLLS showed a high degree of categorical concordance with the commercial methods. Pairwise comparison with each commercial system serving as a reference standard showed 88.9% agreement with MicroScan (two minor errors) and 72.2% agreement with Vitek (five minor errors). FLLS using the BacterioScan system shows promise as a novel method for the rapid and accurate determination of antimicrobial susceptibility.

Full Text

Duke Authors

Cited Authors

  • Hayden, RT; Clinton, LK; Hewitt, C; Koyamatsu, T; Sun, Y; Jamison, G; Perkins, R; Tang, L; Pounds, S; Bankowski, MJ

Published Date

  • November 2016

Published In

Volume / Issue

  • 54 / 11

Start / End Page

  • 2701 - 2706

PubMed ID

  • 27558176

Pubmed Central ID

  • 27558176

Electronic International Standard Serial Number (EISSN)

  • 1098-660X

Digital Object Identifier (DOI)

  • 10.1128/JCM.01475-16


  • eng

Conference Location

  • United States