Disruption of the β1L Isoform of GABP Reverses Glioblastoma Replicative Immortality in a TERT Promoter Mutation-Dependent Manner.

Published

Journal Article

TERT promoter mutations reactivate telomerase, allowing for indefinite telomere maintenance and enabling cellular immortalization. These mutations specifically recruit the multimeric ETS factor GABP, which can form two functionally independent transcription factor species: a dimer or a tetramer. We show that genetic disruption of GABPβ1L (β1L), a tetramer-forming isoform of GABP that is dispensable for normal development, results in TERT silencing in a TERT promoter mutation-dependent manner. Reducing TERT expression by disrupting β1L culminates in telomere loss and cell death exclusively in TERT promoter mutant cells. Orthotopic xenografting of β1L-reduced, TERT promoter mutant glioblastoma cells rendered lower tumor burden and longer overall survival in mice. These results highlight the critical role of GABPβ1L in enabling immortality in TERT promoter mutant glioblastoma.

Full Text

Duke Authors

Cited Authors

  • Mancini, A; Xavier-Magalhães, A; Woods, WS; Nguyen, K-T; Amen, AM; Hayes, JL; Fellmann, C; Gapinske, M; McKinney, AM; Hong, C; Jones, LE; Walsh, KM; Bell, RJA; Doudna, JA; Costa, BM; Song, JS; Perez-Pinera, P; Costello, JF

Published Date

  • September 10, 2018

Published In

Volume / Issue

  • 34 / 3

Start / End Page

  • 513 - 528.e8

PubMed ID

  • 30205050

Pubmed Central ID

  • 30205050

Electronic International Standard Serial Number (EISSN)

  • 1878-3686

Digital Object Identifier (DOI)

  • 10.1016/j.ccell.2018.08.003

Language

  • eng

Conference Location

  • United States