Gustatory receptor neurons in Manduca sexta contain a TrpA1-dependent signaling pathway that integrates taste and temperature.

Published

Journal Article

Temperature modulates the peripheral taste response of many animals, in part by activating transient receptor potential (Trp) cation channels. We hypothesized that temperature would also modulate peripheral taste responses in larval Manduca sexta. We recorded excitatory responses of the lateral and medial styloconic sensilla to chemical stimuli at 14, 22, and 30 °C. The excitatory responses to 5 chemical stimuli-a salt (KCl), 3 sugars (sucrose, glucose, and inositol) and an alkaloid (caffeine)-were unaffected by temperature. In contrast, the excitatory response to the aversive compound, aristolochic acid (AA), increased robustly with temperature. Next, we asked whether TrpA1 mediates the thermally dependent taste response to AA. To this end, we 1) identified a TrpA1 gene in M. sexta; 2) demonstrated expression of TrpA1 in the lateral and medial styloconic sensilla; 3) determined that 2 TrpA1 antagonists (HC-030031 and mecamylamine) inhibit the taste response to AA, but not caffeine; and then 4) established that the thermal dependence of the taste response to AA is blocked by HC-030031. Taken together, our results indicate that TrpA1 serves as a molecular integrator of taste and temperature in M. sexta.

Full Text

Duke Authors

Cited Authors

  • Afroz, A; Howlett, N; Shukla, A; Ahmad, F; Batista, E; Bedard, K; Payne, S; Morton, B; Mansfield, JH; Glendinning, JI

Published Date

  • September 2013

Published In

Volume / Issue

  • 38 / 7

Start / End Page

  • 605 - 617

PubMed ID

  • 23828906

Pubmed Central ID

  • 23828906

Electronic International Standard Serial Number (EISSN)

  • 1464-3553

International Standard Serial Number (ISSN)

  • 0379-864X

Digital Object Identifier (DOI)

  • 10.1093/chemse/bjt032

Language

  • eng