IL-6 and TNF-alpha expression in, and release from, contracting human skeletal muscle.

Journal Article (Clinical Trial;Journal Article)

The aim of the present study was to examine whether IL-6 and TNF-alpha are expressed in, and released from, human skeletal muscle during exercise. We hypothesized that the skeletal muscle will release IL-6, but not TNF-alpha, during exercise because of previous observations that TNF-alpha negatively affects glucose uptake in skeletal muscle. Six healthy, male subjects performed 180 min of two-legged knee-extensor exercise. Muscle samples were obtained from the vastus lateralis of one limb. In addition, blood samples were obtained from a femoral artery and vein. Plasma was analyzed for IL-6 and TNF-alpha. We detected both IL-6 and TNF-alpha mRNA in resting muscle samples, and whereas IL-6 increased (P < 0.05) approximately 100-fold throughout exercise, no significant increase in TNF-alpha mRNA was observed. Arterial plasma TNF-alpha did not increase during exercise. Furthermore, there was no net release of TNF-alpha either before or during exercise. In contrast, IL-6 increased throughout exercise in arterial plasma, and a net IL-6 release from the contracting limb was observed after 120 min of exercise (P < 0.05).

Full Text

Duke Authors

Cited Authors

  • Steensberg, A; Keller, C; Starkie, RL; Osada, T; Febbraio, MA; Pedersen, BK

Published Date

  • December 2002

Published In

Volume / Issue

  • 283 / 6

Start / End Page

  • E1272 - E1278

PubMed ID

  • 12388119

International Standard Serial Number (ISSN)

  • 0193-1849

Digital Object Identifier (DOI)

  • 10.1152/ajpendo.00255.2002


  • eng

Conference Location

  • United States