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Doxycycline alters collagen composition following ventral hernia repair.

Publication ,  Journal Article
Tharappel, JC; Harris, JW; Totten, C; Zwischenberger, BA; Roth, JS
Published in: Surg Endosc
April 2017

BACKGROUND: Doxycycline, a nonspecific metalloproteinase (MMP) inhibitor, has been demonstrated to impact the strength of the polypropylene (PP) mesh-repaired hernia with an increase in the deposition of collagen type 1. The impact of doxycycline with porcine acellular dermal matrices (PADM) is unknown; therefore, we evaluated the impact of doxycycline administration upon hernia repair with PP and PADM mesh. METHODS: Sprague-Dawley rats weighing ~400 g underwent laparotomy with creation of a midline ventral hernia. After a 27-day recovery, animals were randomly assigned to four groups of eight and underwent intraperitoneal underlay hernia repair with either PP or PADM. Groups were assigned to daily normal saline (S) or daily doxycycline in normal saline 10 mg/kg (D) via oral gavage for 8 weeks beginning 24 h preoperatively. Animals were euthanized at 8 weeks and underwent tensiometric testing of the abdominal wall and western blot analyses for collagen subtypes and MMPs. RESULTS: Thirty-two animals underwent successful hernia creation and repair with either PADM or PP. At 8 weeks, 15 of 16 PP-implanted animals survived with only 12 of 16 PADM-implanted animals surviving. There were no differences in the mesh to fascial interface tensiometric strength between groups. Densitometric counts in the PADM-D group demonstrated increased collagen type 1 compared to PP-S (PADM-D [1286.5], PADM-S [906.9], PP-S [700.4], p = 0.037) and decreased collagen type 3 compared to PP-S (PADM-D [7446.9], PADM-S [8507.6], PP-S [11,297.1], p = 0.01). MMP-9 levels were increased in PADM-D (PP-S vs. PADM-D, p = 0.04), while MMP-2 levels were similar between PADM-D and PADM-S, respectively. CONCLUSIONS: Collagen type 1 deposition at the mesh to fascial interface is enhanced following administration of doxycycline in ventral hernia repairs with porcine acellular dermal matrices. Doxycycline administration may have implications for enhancing hernia repair outcomes using biologic mesh.

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Published In

Surg Endosc

DOI

EISSN

1432-2218

Publication Date

April 2017

Volume

31

Issue

4

Start / End Page

1659 / 1666

Location

Germany

Related Subject Headings

  • Wound Healing
  • Surgical Mesh
  • Surgery
  • Rats, Sprague-Dawley
  • Rats
  • Random Allocation
  • Matrix Metalloproteinase 9
  • Matrix Metalloproteinase 2
  • Herniorrhaphy
  • Hernia, Ventral
 

Citation

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Tharappel, J. C., Harris, J. W., Totten, C., Zwischenberger, B. A., & Roth, J. S. (2017). Doxycycline alters collagen composition following ventral hernia repair. Surg Endosc, 31(4), 1659–1666. https://doi.org/10.1007/s00464-016-5155-8
Tharappel, Job C., Jennifer W. Harris, Crystal Totten, Brittany A. Zwischenberger, and John S. Roth. “Doxycycline alters collagen composition following ventral hernia repair.Surg Endosc 31, no. 4 (April 2017): 1659–66. https://doi.org/10.1007/s00464-016-5155-8.
Tharappel JC, Harris JW, Totten C, Zwischenberger BA, Roth JS. Doxycycline alters collagen composition following ventral hernia repair. Surg Endosc. 2017 Apr;31(4):1659–66.
Tharappel, Job C., et al. “Doxycycline alters collagen composition following ventral hernia repair.Surg Endosc, vol. 31, no. 4, Apr. 2017, pp. 1659–66. Pubmed, doi:10.1007/s00464-016-5155-8.
Tharappel JC, Harris JW, Totten C, Zwischenberger BA, Roth JS. Doxycycline alters collagen composition following ventral hernia repair. Surg Endosc. 2017 Apr;31(4):1659–1666.
Journal cover image

Published In

Surg Endosc

DOI

EISSN

1432-2218

Publication Date

April 2017

Volume

31

Issue

4

Start / End Page

1659 / 1666

Location

Germany

Related Subject Headings

  • Wound Healing
  • Surgical Mesh
  • Surgery
  • Rats, Sprague-Dawley
  • Rats
  • Random Allocation
  • Matrix Metalloproteinase 9
  • Matrix Metalloproteinase 2
  • Herniorrhaphy
  • Hernia, Ventral