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Doxycycline shows dose-dependent changes in hernia repair strength after mesh repair.

Publication ,  Journal Article
Tharappel, JC; Harris, JW; Zwischenberger, BA; Levy, SM; Puleo, DA; Roth, JS
Published in: Surg Endosc
May 2016

BACKGROUND: Ventral hernia is a commonly occurring surgical problem. Our earlier studies have shown that a 30 mg/kg dose of doxycycline can significantly impact the strength of polypropylene (PP) mesh in a rat hernia repair model at 6 and 12 weeks. The objective of the present study was to investigate the dose dependence of doxycycline treatment on hernia repair strengths in rats. STUDY DESIGN: Fifty-six Sprague-Dawley rats underwent hernia repair with either PP mesh (n = 28) or sutures only (primary; n = 28); both groups were further divided into four doxycycline groups of seven animals each: control (0 mg/kg), low (3 mg/kg), medium (10 mg/kg), and high (30 mg/kg). One day before hernia repair surgery, animals received doxycycline doses by gavage and continued receiving daily until euthanasia. After 8 weeks, rats were euthanized and tissue samples from hernia repaired area were collected and analyzed for tensile strength using a tensiometer (Instron, Canton, MA, USA), while MMPs 2, 3, and 9, and collagen type 1 and 3 were analyzed by western blotting. RESULTS: In mesh-repaired animals, medium and high doxycycline dose repaired mesh fascia interface (MFI) showed significant increase in tensile strength when compared to control. In the primary repaired animals, there was no significant difference in MFI tensile strength in any dose group. In medium-dose MFI, there was a significant reduction in MMPs 2, 3, and 9. In this animal group, MFI showed significant increase in collagen 1 and significant reduction in collagen type 3 when compared to control. CONCLUSION: It is possible to improve the strength of mesh-repaired tissue by administering a significantly lower dose of the drug, which has implications for translation of the findings.

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Published In

Surg Endosc

DOI

EISSN

1432-2218

Publication Date

May 2016

Volume

30

Issue

5

Start / End Page

2016 / 2021

Location

Germany

Related Subject Headings

  • Tensile Strength
  • Sutures
  • Surgical Mesh
  • Surgery
  • Rats, Sprague-Dawley
  • Rats
  • Prostheses and Implants
  • Polypropylenes
  • Matrix Metalloproteinase 9
  • Matrix Metalloproteinase 3
 

Citation

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Tharappel, J. C., Harris, J. W., Zwischenberger, B. A., Levy, S. M., Puleo, D. A., & Roth, J. S. (2016). Doxycycline shows dose-dependent changes in hernia repair strength after mesh repair. Surg Endosc, 30(5), 2016–2021. https://doi.org/10.1007/s00464-015-4434-0
Tharappel, Job C., Jennifer W. Harris, Brittany A. Zwischenberger, Salomon M. Levy, David A. Puleo, and J Scott Roth. “Doxycycline shows dose-dependent changes in hernia repair strength after mesh repair.Surg Endosc 30, no. 5 (May 2016): 2016–21. https://doi.org/10.1007/s00464-015-4434-0.
Tharappel JC, Harris JW, Zwischenberger BA, Levy SM, Puleo DA, Roth JS. Doxycycline shows dose-dependent changes in hernia repair strength after mesh repair. Surg Endosc. 2016 May;30(5):2016–21.
Tharappel, Job C., et al. “Doxycycline shows dose-dependent changes in hernia repair strength after mesh repair.Surg Endosc, vol. 30, no. 5, May 2016, pp. 2016–21. Pubmed, doi:10.1007/s00464-015-4434-0.
Tharappel JC, Harris JW, Zwischenberger BA, Levy SM, Puleo DA, Roth JS. Doxycycline shows dose-dependent changes in hernia repair strength after mesh repair. Surg Endosc. 2016 May;30(5):2016–2021.
Journal cover image

Published In

Surg Endosc

DOI

EISSN

1432-2218

Publication Date

May 2016

Volume

30

Issue

5

Start / End Page

2016 / 2021

Location

Germany

Related Subject Headings

  • Tensile Strength
  • Sutures
  • Surgical Mesh
  • Surgery
  • Rats, Sprague-Dawley
  • Rats
  • Prostheses and Implants
  • Polypropylenes
  • Matrix Metalloproteinase 9
  • Matrix Metalloproteinase 3