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Extracellular matrix proteins and carcinoembryonic antigen-related cell adhesion molecules characterize pancreatic duct fluid exosomes in patients with pancreatic cancer.

Publication ,  Journal Article
Zheng, J; Hernandez, JM; Doussot, A; Bojmar, L; Zambirinis, CP; Costa-Silva, B; van Beek, EJAH; Mark, MT; Molina, H; Askan, G; Basturk, O ...
Published in: HPB (Oxford)
July 2018

BACKGROUND: Exosomes are nanovesicles that have been shown to mediate carcinogenesis in pancreatic ductal adenocarcinoma (PDAC). Given the direct communication of pancreatic duct fluid with the tumor and its relative accessibility, we aimed to determine the feasibility of isolating and characterizing exosomes from pancreatic duct fluid. METHODS: Pancreatic duct fluid was collected from 26 patients with PDAC (n = 13), intraductal papillary mucinous neoplasm (IPMN) (n = 8) and other benign pancreatic diseases (n = 5) at resection. Exosomes were isolated by serial ultracentrifugation, proteins were identified by mass spectrometry, and their expression was evaluated by immunohistochemistry. RESULTS: Exosomes were isolated from all specimens with a mean concentration of 5.9 ± 1 × 108 particles/mL and most frequent size of 138 ± 9 nm. Among the top 35 proteins that were significantly associated with PDAC, multiple carcinoembryonic antigen-related cell adhesion molecules (CEACAMs) and extracellular matrix (ECM) proteins were identified. Interestingly, CEACAM 1/5 expression by immunohistochemistry was seen only on tumor epithelia whereas tenascin C positivity was restricted to stroma, suggesting that both tumor and stromal cells contributed to exosomes. CONCLUSION: This is the first study showing that exosome isolation is feasible from pancreatic duct fluid, and that exosomal proteins may be utilized to diagnose patients with PDAC.

Duke Scholars

Published In

HPB (Oxford)

DOI

EISSN

1477-2574

Publication Date

July 2018

Volume

20

Issue

7

Start / End Page

597 / 604

Location

England

Related Subject Headings

  • Ultracentrifugation
  • Surgery
  • Predictive Value of Tests
  • Pilot Projects
  • Pancreatic Neoplasms
  • Pancreatic Juice
  • Pancreatic Intraductal Neoplasms
  • Pancreatic Ducts
  • Middle Aged
  • Mass Spectrometry
 

Citation

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Zheng, J., Hernandez, J. M., Doussot, A., Bojmar, L., Zambirinis, C. P., Costa-Silva, B., … Jarnagin, W. R. (2018). Extracellular matrix proteins and carcinoembryonic antigen-related cell adhesion molecules characterize pancreatic duct fluid exosomes in patients with pancreatic cancer. HPB (Oxford), 20(7), 597–604. https://doi.org/10.1016/j.hpb.2017.12.010
Zheng, Jian, Jonathan M. Hernandez, Alexandre Doussot, Linda Bojmar, Constantinos P. Zambirinis, Bruno Costa-Silva, Elke J. A. H. van Beek, et al. “Extracellular matrix proteins and carcinoembryonic antigen-related cell adhesion molecules characterize pancreatic duct fluid exosomes in patients with pancreatic cancer.HPB (Oxford) 20, no. 7 (July 2018): 597–604. https://doi.org/10.1016/j.hpb.2017.12.010.
Zheng J, Hernandez JM, Doussot A, Bojmar L, Zambirinis CP, Costa-Silva B, et al. Extracellular matrix proteins and carcinoembryonic antigen-related cell adhesion molecules characterize pancreatic duct fluid exosomes in patients with pancreatic cancer. HPB (Oxford). 2018 Jul;20(7):597–604.
Zheng, Jian, et al. “Extracellular matrix proteins and carcinoembryonic antigen-related cell adhesion molecules characterize pancreatic duct fluid exosomes in patients with pancreatic cancer.HPB (Oxford), vol. 20, no. 7, July 2018, pp. 597–604. Pubmed, doi:10.1016/j.hpb.2017.12.010.
Zheng J, Hernandez JM, Doussot A, Bojmar L, Zambirinis CP, Costa-Silva B, van Beek EJAH, Mark MT, Molina H, Askan G, Basturk O, Gonen M, Kingham TP, Allen PJ, D’Angelica MI, DeMatteo RP, Lyden D, Jarnagin WR. Extracellular matrix proteins and carcinoembryonic antigen-related cell adhesion molecules characterize pancreatic duct fluid exosomes in patients with pancreatic cancer. HPB (Oxford). 2018 Jul;20(7):597–604.
Journal cover image

Published In

HPB (Oxford)

DOI

EISSN

1477-2574

Publication Date

July 2018

Volume

20

Issue

7

Start / End Page

597 / 604

Location

England

Related Subject Headings

  • Ultracentrifugation
  • Surgery
  • Predictive Value of Tests
  • Pilot Projects
  • Pancreatic Neoplasms
  • Pancreatic Juice
  • Pancreatic Intraductal Neoplasms
  • Pancreatic Ducts
  • Middle Aged
  • Mass Spectrometry