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Comparison of surgical and endoscopic sample collection for pancreatic cyst fluid biomarker identification.

Publication ,  Journal Article
Partyka, K; McDonald, M; Maupin, KA; Brand, R; Kwon, R; Simeone, DM; Allen, P; Haab, BB
Published in: J Proteome Res
May 4, 2012

Significant efforts are underway to develop new biomarkers from pancreatic cyst fluid. Previous research has made use of cyst fluid collected from surgically removed cysts, but the clinical implementation of biomarkers would use cyst fluid collected by endoscopic ultrasound-guided, fine-needle aspiration (EUS-FNA). The purpose of this study was to investigate the clinical applicability of cyst fluid research obtained using surgical specimens. Matched pairs of operating-room collected (OR) and EUS-FNA samples from 12 patients were evaluated for the levels of three previously described biomarkers, CA 19-9, CEA, and glycan levels detected by wheat germ agglutinin on MUC5AC (MUC5AC-WGA). CA 19-9 and MUC5AC-WGA correlated well between the sample types, although CEA was more variable between the sample types for certain patients. The variability was not due to the time delay between EUS-FNA and OR collection or differences in total protein concentrations but may be caused by contamination of the cyst fluid with blood proteins. The classification of each patient based on thresholds for each marker was perfectly consistent between sample types for CA 19-9 and MUC5AC-WGA and mostly consistent for CEA. Therefore, results obtained using OR-collected pancreatic cyst fluid samples should reliably transfer to the clinical setting using EUS-FNA samples.

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Published In

J Proteome Res

DOI

EISSN

1535-3907

Publication Date

May 4, 2012

Volume

11

Issue

5

Start / End Page

2904 / 2911

Location

United States

Related Subject Headings

  • Specimen Handling
  • Reproducibility of Results
  • Pancreatic Cyst
  • Operating Rooms
  • Mucin 5AC
  • Humans
  • Endosonography
  • Cyst Fluid
  • CA-19-9 Antigen
  • Blood Proteins
 

Citation

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Partyka, K., McDonald, M., Maupin, K. A., Brand, R., Kwon, R., Simeone, D. M., … Haab, B. B. (2012). Comparison of surgical and endoscopic sample collection for pancreatic cyst fluid biomarker identification. J Proteome Res, 11(5), 2904–2911. https://doi.org/10.1021/pr2012736
Partyka, Katie, Mitchell McDonald, Kevin A. Maupin, Randall Brand, Richard Kwon, Diane M. Simeone, Peter Allen, and Brian B. Haab. “Comparison of surgical and endoscopic sample collection for pancreatic cyst fluid biomarker identification.J Proteome Res 11, no. 5 (May 4, 2012): 2904–11. https://doi.org/10.1021/pr2012736.
Partyka K, McDonald M, Maupin KA, Brand R, Kwon R, Simeone DM, et al. Comparison of surgical and endoscopic sample collection for pancreatic cyst fluid biomarker identification. J Proteome Res. 2012 May 4;11(5):2904–11.
Partyka, Katie, et al. “Comparison of surgical and endoscopic sample collection for pancreatic cyst fluid biomarker identification.J Proteome Res, vol. 11, no. 5, May 2012, pp. 2904–11. Pubmed, doi:10.1021/pr2012736.
Partyka K, McDonald M, Maupin KA, Brand R, Kwon R, Simeone DM, Allen P, Haab BB. Comparison of surgical and endoscopic sample collection for pancreatic cyst fluid biomarker identification. J Proteome Res. 2012 May 4;11(5):2904–2911.
Journal cover image

Published In

J Proteome Res

DOI

EISSN

1535-3907

Publication Date

May 4, 2012

Volume

11

Issue

5

Start / End Page

2904 / 2911

Location

United States

Related Subject Headings

  • Specimen Handling
  • Reproducibility of Results
  • Pancreatic Cyst
  • Operating Rooms
  • Mucin 5AC
  • Humans
  • Endosonography
  • Cyst Fluid
  • CA-19-9 Antigen
  • Blood Proteins