Comparison of surgical and endoscopic sample collection for pancreatic cyst fluid biomarker identification.

Published

Journal Article

Significant efforts are underway to develop new biomarkers from pancreatic cyst fluid. Previous research has made use of cyst fluid collected from surgically removed cysts, but the clinical implementation of biomarkers would use cyst fluid collected by endoscopic ultrasound-guided, fine-needle aspiration (EUS-FNA). The purpose of this study was to investigate the clinical applicability of cyst fluid research obtained using surgical specimens. Matched pairs of operating-room collected (OR) and EUS-FNA samples from 12 patients were evaluated for the levels of three previously described biomarkers, CA 19-9, CEA, and glycan levels detected by wheat germ agglutinin on MUC5AC (MUC5AC-WGA). CA 19-9 and MUC5AC-WGA correlated well between the sample types, although CEA was more variable between the sample types for certain patients. The variability was not due to the time delay between EUS-FNA and OR collection or differences in total protein concentrations but may be caused by contamination of the cyst fluid with blood proteins. The classification of each patient based on thresholds for each marker was perfectly consistent between sample types for CA 19-9 and MUC5AC-WGA and mostly consistent for CEA. Therefore, results obtained using OR-collected pancreatic cyst fluid samples should reliably transfer to the clinical setting using EUS-FNA samples.

Full Text

Duke Authors

Cited Authors

  • Partyka, K; McDonald, M; Maupin, KA; Brand, R; Kwon, R; Simeone, DM; Allen, P; Haab, BB

Published Date

  • May 4, 2012

Published In

Volume / Issue

  • 11 / 5

Start / End Page

  • 2904 - 2911

PubMed ID

  • 22439797

Pubmed Central ID

  • 22439797

Electronic International Standard Serial Number (EISSN)

  • 1535-3907

Digital Object Identifier (DOI)

  • 10.1021/pr2012736

Language

  • eng

Conference Location

  • United States