Serum biomarker panels for the detection of pancreatic cancer.

Published

Journal Article

Serum-biomarker based screening for pancreatic cancer could greatly improve survival in appropriately targeted high-risk populations.Eighty-three circulating proteins were analyzed in sera of patients diagnosed with pancreatic ductal adenocarcinoma (PDAC, n = 333), benign pancreatic conditions (n = 144), and healthy control individuals (n = 227). Samples from each group were split randomly into training and blinded validation sets prior to analysis. A Metropolis algorithm with Monte Carlo simulation (MMC) was used to identify discriminatory biomarker panels in the training set. Identified panels were evaluated in the validation set and in patients diagnosed with colon (n = 33), lung (n = 62), and breast (n = 108) cancers.Several robust profiles of protein alterations were present in sera of PDAC patients compared to the Healthy and Benign groups. In the training set (n = 160 PDAC, 74 Benign, 107 Healthy), the panel of CA 19-9, ICAM-1, and OPG discriminated PDAC patients from Healthy controls with a sensitivity/specificity (SN/SP) of 88/90%, while the panel of CA 19-9, CEA, and TIMP-1 discriminated PDAC patients from Benign subjects with an SN/SP of 76/90%. In an independent validation set (n = 173 PDAC, 70 Benign, 120 Healthy), the panel of CA 19-9, ICAM-1 and OPG demonstrated an SN/SP of 78/94% while the panel of CA19-9, CEA, and TIMP-1 demonstrated an SN/SP of 71/89%. The CA19-9, ICAM-1, OPG panel is selective for PDAC and does not recognize breast (SP = 100%), lung (SP = 97%), or colon (SP = 97%) cancer.The PDAC-specific biomarker panels identified in this investigation warrant additional clinical validation to determine their role in screening targeted high-risk populations.

Full Text

Duke Authors

Cited Authors

  • Brand, RE; Nolen, BM; Zeh, HJ; Allen, PJ; Eloubeidi, MA; Goldberg, M; Elton, E; Arnoletti, JP; Christein, JD; Vickers, SM; Langmead, CJ; Landsittel, DP; Whitcomb, DC; Grizzle, WE; Lokshin, AE

Published Date

  • February 2011

Published In

Volume / Issue

  • 17 / 4

Start / End Page

  • 805 - 816

PubMed ID

  • 21325298

Pubmed Central ID

  • 21325298

International Standard Serial Number (ISSN)

  • 1078-0432

Digital Object Identifier (DOI)

  • 10.1158/1078-0432.CCR-10-0248

Language

  • eng