Involvement of urokinase-type plasminogen activator in acantholysis induced by pemphigus IgG.

Published

Journal Article

Pemphigus IgG induces acantholysis in skin organ culture without the involvement of complement. Urokinase-type plasminogen activator, a proteolytic enzyme, has been implicated in the development of acantholysis. To test this hypothesis, we prepared a rabbit anti-urokinase antibody, which inhibited the plasminogen activator activity in normal human epidermis and in cultured keratinocytes. When added to skin organ cultures along with pemphigus IgG, anti-urokinase IgG completely prevented the development of acantholysis. Normal or preimmune rabbit IgG had no effect on pemphigus IgG-induced acantholysis. Plasminogen activator converts the zymogen plasminogen to its active form plasmin, a broad specificity serine proteinase. When high concentrations of plasminogen alone were added to skin organ culture, acantholysis of the pemphigus foliaceous type was induced. Anti-urokinase antibody also inhibited plasminogen-induced acantholysis. These results strongly support a pivotal role for plasminogen activator in the development of acantholysis.

Full Text

Duke Authors

Cited Authors

  • Morioka, S; Lazarus, GS; Jensen, PJ

Published Date

  • November 1987

Published In

Volume / Issue

  • 89 / 5

Start / End Page

  • 474 - 477

PubMed ID

  • 3117905

Pubmed Central ID

  • 3117905

Electronic International Standard Serial Number (EISSN)

  • 1523-1747

International Standard Serial Number (ISSN)

  • 0022-202X

Digital Object Identifier (DOI)

  • 10.1111/1523-1747.ep12460937

Language

  • eng