The dual enzyme LRRK2 hydrolyzes GTP in both its GTPase and kinase domains in vitro.

Journal Article (Journal Article)

The evolutionarily conserved enzyme encoded by the leucine-rich repeat kinase 2 gene, LRRK2, harbors both a Rab-like GTPase domain and a serine/threonine protein kinase domain. Pathogenic mutations in either the GTPase or kinase domain can cause neurodegeneration and Parkinson disease. No high-resolution structure of the human LRRK2 kinase domain is available but the most common mutation, G2019S in the kinase domain, is predicted to alter the ATP-binding pocket structure and interaction with divalent cations. Here we find that the manganese-bound kinase domain acquires a robust ability to utilize both GTP as well as ATP in autophosphorylation of the GTPase domain and phosphorylation of peptide substrates in vitro. The G2019S LRRK2 mutation increases the efficiency of GTP-mediated kinase activity ten-fold compared to WT LRRK2 activity. Moreover, GTP-dependent phosphorylation alters autophosphorylation-site preference in vitro. While additional studies are required to determine the physiological relevance of these observations, LRRK2 is one of the only known kinases to be able to utilize GTP as a phospho-donor at physiological levels in vitro, and thus one of the only known proteins to be able to hydrolyze GTP in two distinct domains within the same protein.

Full Text

Duke Authors

Cited Authors

  • Liu, Z; West, AB

Published Date

  • March 2017

Published In

Volume / Issue

  • 1865 / 3

Start / End Page

  • 274 - 280

PubMed ID

  • 27939437

Pubmed Central ID

  • PMC5278637

International Standard Serial Number (ISSN)

  • 1570-9639

Digital Object Identifier (DOI)

  • 10.1016/j.bbapap.2016.12.001


  • eng

Conference Location

  • Netherlands