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Parkin is not regulated by the unfolded protein response in human neuroblastoma cells.

Publication ,  Journal Article
West, AB; Gonzalez-de-Chavez, F; Wilkes, K; O'Farrell, C; Farrer, MJ
Published in: Neurosci Lett
May 1, 2003

Mutations in the parkin gene cause the majority of cases of familial-linked Parkinson's disease, and mounting evidence suggests that parkin may play a role in idiopathic disease. Previous reports suggest that parkin may respond to and relieve, via E3-ligase activity, cellular stress at the endoplasmic reticulum caused by the accumulation of unfolded proteins. However, parkin's relationship to the mammalian unfolded protein response is unclear. Here, we comprehensively evaluate endogenous parkin in SH-SY5Y neuroblastomas at the promoter, RNA, and protein levels in response to unfolded protein stress induced by tunicamycin. While we find strong up-regulation of genes linked to the unfolded protein stress pathway, we detect no significant changes in parkin. These data suggest a lack of association between parkin and the unfolded protein response in SH-SY5Y cells.

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Published In

Neurosci Lett

DOI

ISSN

0304-3940

Publication Date

May 1, 2003

Volume

341

Issue

2

Start / End Page

139 / 142

Location

Ireland

Related Subject Headings

  • Ubiquitin-Protein Ligases
  • Tunicamycin
  • Transfection
  • Time Factors
  • Reverse Transcriptase Polymerase Chain Reaction
  • RNA, Messenger
  • Protein Folding
  • Promoter Regions, Genetic
  • Peptide Fragments
  • Neuroblastoma
 

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West, A. B., Gonzalez-de-Chavez, F., Wilkes, K., O’Farrell, C., & Farrer, M. J. (2003). Parkin is not regulated by the unfolded protein response in human neuroblastoma cells. Neurosci Lett, 341(2), 139–142. https://doi.org/10.1016/s0304-3940(03)00188-5
West, Andrew B., Fanny Gonzalez-de-Chavez, Kristen Wilkes, Casey O’Farrell, and Matthew J. Farrer. “Parkin is not regulated by the unfolded protein response in human neuroblastoma cells.Neurosci Lett 341, no. 2 (May 1, 2003): 139–42. https://doi.org/10.1016/s0304-3940(03)00188-5.
West AB, Gonzalez-de-Chavez F, Wilkes K, O’Farrell C, Farrer MJ. Parkin is not regulated by the unfolded protein response in human neuroblastoma cells. Neurosci Lett. 2003 May 1;341(2):139–42.
West, Andrew B., et al. “Parkin is not regulated by the unfolded protein response in human neuroblastoma cells.Neurosci Lett, vol. 341, no. 2, May 2003, pp. 139–42. Pubmed, doi:10.1016/s0304-3940(03)00188-5.
West AB, Gonzalez-de-Chavez F, Wilkes K, O’Farrell C, Farrer MJ. Parkin is not regulated by the unfolded protein response in human neuroblastoma cells. Neurosci Lett. 2003 May 1;341(2):139–142.
Journal cover image

Published In

Neurosci Lett

DOI

ISSN

0304-3940

Publication Date

May 1, 2003

Volume

341

Issue

2

Start / End Page

139 / 142

Location

Ireland

Related Subject Headings

  • Ubiquitin-Protein Ligases
  • Tunicamycin
  • Transfection
  • Time Factors
  • Reverse Transcriptase Polymerase Chain Reaction
  • RNA, Messenger
  • Protein Folding
  • Promoter Regions, Genetic
  • Peptide Fragments
  • Neuroblastoma