Parkin is not regulated by the unfolded protein response in human neuroblastoma cells.

Published

Journal Article

Mutations in the parkin gene cause the majority of cases of familial-linked Parkinson's disease, and mounting evidence suggests that parkin may play a role in idiopathic disease. Previous reports suggest that parkin may respond to and relieve, via E3-ligase activity, cellular stress at the endoplasmic reticulum caused by the accumulation of unfolded proteins. However, parkin's relationship to the mammalian unfolded protein response is unclear. Here, we comprehensively evaluate endogenous parkin in SH-SY5Y neuroblastomas at the promoter, RNA, and protein levels in response to unfolded protein stress induced by tunicamycin. While we find strong up-regulation of genes linked to the unfolded protein stress pathway, we detect no significant changes in parkin. These data suggest a lack of association between parkin and the unfolded protein response in SH-SY5Y cells.

Full Text

Duke Authors

Cited Authors

  • West, AB; Gonzalez-de-Chavez, F; Wilkes, K; O'Farrell, C; Farrer, MJ

Published Date

  • May 1, 2003

Published In

Volume / Issue

  • 341 / 2

Start / End Page

  • 139 - 142

PubMed ID

  • 12686385

Pubmed Central ID

  • 12686385

International Standard Serial Number (ISSN)

  • 0304-3940

Digital Object Identifier (DOI)

  • 10.1016/s0304-3940(03)00188-5

Language

  • eng

Conference Location

  • Ireland