Loss of LDAH associated with prostate cancer and hearing loss.

Published

Journal Article

Great strides in gene discovery have been made using a multitude of methods to associate phenotypes with genetic variants, but there still remains a substantial gap between observed symptoms and identified genetic defects. Herein, we use the convergence of various genetic and genomic techniques to investigate the underpinnings of a constellation of phenotypes that include prostate cancer (PCa) and sensorineural hearing loss (SNHL) in a human subject. Through interrogation of the subject's de novo, germline, balanced chromosomal translocation, we first identify a correlation between his disorders and a poorly annotated gene known as lipid droplet associated hydrolase (LDAH). Using data repositories of both germline and somatic variants, we identify convergent genomic evidence that substantiates a correlation between loss of LDAH and PCa. This correlation is validated through both in vitro and in vivo models that show loss of LDAH results in increased risk of PCa and, to a lesser extent, SNHL. By leveraging convergent evidence in emerging genomic data, we hypothesize that loss of LDAH is involved in PCa and other phenotypes observed in support of a genotype-phenotype association in an n-of-one human subject.

Full Text

Duke Authors

Cited Authors

  • Currall, BB; Chen, M; Sallari, RC; Cotter, M; Wong, KE; Robertson, NG; Penney, KL; Lunardi, A; Reschke, M; Hickox, AE; Yin, Y; Wong, GT; Fung, J; Brown, KK; Williamson, RE; Sinnott-Armstrong, NA; Kammin, T; Ivanov, A; Zepeda-Mendoza, CJ; Shen, J; Quade, BJ; Signoretti, S; Arnos, KS; Banks, AS; Patsopoulos, N; Liberman, MC; Kellis, M; Pandolfi, PP; Morton, CC

Published Date

  • December 15, 2018

Published In

Volume / Issue

  • 27 / 24

Start / End Page

  • 4194 - 4203

PubMed ID

  • 30169630

Pubmed Central ID

  • 30169630

Electronic International Standard Serial Number (EISSN)

  • 1460-2083

Digital Object Identifier (DOI)

  • 10.1093/hmg/ddy310

Language

  • eng

Conference Location

  • England