IDLV-HIV-1 Env vaccination in non-human primates induces affinity maturation of antigen-specific memory B cells.

Published online

Journal Article

HIV continues to be a major global health issue. In spite of successful prevention interventions and treatment methods, the development of an HIV vaccine remains a major priority for the field and would be the optimal strategy to prevent new infections. We showed previously that a single immunization with a SIV-based integrase-defective lentiviral vector (IDLV) expressing the 1086.C HIV-1-envelope induced durable, high-magnitude immune responses in non-human primates (NHPs). In this study, we have further characterized the humoral responses by assessing antibody affinity maturation and antigen-specific memory B-cell persistence in two vaccinated macaques. These animals were also boosted with IDLV expressing the heterologous 1176.C HIV-1-Env to determine if neutralization breadth could be increased, followed by evaluation of the injection sites to assess IDLV persistence. IDLV-Env immunization was associated with persistence of the vector DNA for up to 6 months post immunization and affinity maturation of antigen-specific memory B cells.

Full Text

Duke Authors

Cited Authors

  • Blasi, M; Negri, D; LaBranche, C; Alam, SM; Baker, EJ; Brunner, EC; Gladden, MA; Michelini, Z; Vandergrift, NA; Wiehe, KJ; Parks, R; Shen, X; Bonsignori, M; Tomaras, GD; Ferrari, G; Montefiori, DC; Santra, S; Haynes, BF; Moody, MA; Cara, A; Klotman, ME

Published Date

  • 2018

Published In

Volume / Issue

  • 1 /

Start / End Page

  • 134 -

PubMed ID

  • 30272013

Pubmed Central ID

  • 30272013

Electronic International Standard Serial Number (EISSN)

  • 2399-3642

Digital Object Identifier (DOI)

  • 10.1038/s42003-018-0131-6

Language

  • eng

Conference Location

  • England