Non-proteolytic activity of 19S proteasome subunit RPT-6 regulates GATA transcription during response to infection.

Published online

Journal Article

GATA transcription factors play a crucial role in the regulation of immune functions across metazoans. In Caenorhabditis elegans, the GATA transcription factor ELT-2 is involved in the control of not only infections but also recovery after an infection. We identified RPT-6, part of the 19S proteasome subunit, as an ELT-2 binding partner that is required for the proper expression of genes required for both immunity against bacterial infections and recovery after infection. We found that the intact ATPase domain of RPT-6 is required for the interaction and that inhibition of rpt-6 affected the expression of ELT-2-controlled genes, preventing the appropriate immune response against Pseudomonas aeruginosa and recovery from infection by the pathogen. Further studies indicated that SKN-1, which is an Nrf transcription factor involved in the response to oxidative stress and infection, is activated by inhibition of rpt-6. Our results indicate that RPT-6 interacts with ELT-2 in vivo to control the expression of immune genes in a manner that is likely independent of the proteolytic activity of the proteasome.

Full Text

Cited Authors

  • Olaitan, AO; Aballay, A

Published Date

  • September 2018

Published In

Volume / Issue

  • 14 / 9

Start / End Page

  • e1007693 -

PubMed ID

  • 30265660

Pubmed Central ID

  • 30265660

Electronic International Standard Serial Number (EISSN)

  • 1553-7404

Digital Object Identifier (DOI)

  • 10.1371/journal.pgen.1007693

Language

  • eng

Conference Location

  • United States