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Single-Cell Analysis of Quiescent HIV Infection Reveals Host Transcriptional Profiles that Regulate Proviral Latency.

Publication ,  Journal Article
Bradley, T; Ferrari, G; Haynes, BF; Margolis, DM; Browne, EP
Published in: Cell Rep
October 2, 2018

A detailed understanding of the mechanisms that establish or maintain the latent reservoir of HIV will guide approaches to eliminate persistent infection. We used a cell line and primary cell models of HIV latency to investigate viral RNA (vRNA) expression and the role of the host transcriptome using single-cell approaches. Single-cell vRNA quantitation identified distinct populations of cells expressing various levels of vRNA, including completely silent populations. Strikingly, single-cell RNA-seq of latently infected primary cells demonstrated that HIV downregulation occurred in diverse transcriptomic environments but was significantly associated with expression of a specific set of cellular genes. In particular, latency was more frequent in cells expressing a transcriptional signature that included markers of naive and central memory T cells. These data reveal that expression of HIV proviruses within the latent reservoir are influenced by the host cell transcriptional program. Therapeutic modulation of these programs may reverse or enforce HIV latency.

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Published In

Cell Rep

DOI

EISSN

2211-1247

Publication Date

October 2, 2018

Volume

25

Issue

1

Start / End Page

107 / 117.e3

Location

United States

Related Subject Headings

  • Virus Latency
  • Transcription, Genetic
  • Single-Cell Analysis
  • RNA, Viral
  • Proviruses
  • Jurkat Cells
  • Humans
  • Host Microbial Interactions
  • HIV Infections
  • HIV
 

Citation

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Bradley, T., Ferrari, G., Haynes, B. F., Margolis, D. M., & Browne, E. P. (2018). Single-Cell Analysis of Quiescent HIV Infection Reveals Host Transcriptional Profiles that Regulate Proviral Latency. Cell Rep, 25(1), 107-117.e3. https://doi.org/10.1016/j.celrep.2018.09.020
Bradley, Todd, Guido Ferrari, Barton F. Haynes, David M. Margolis, and Edward P. Browne. “Single-Cell Analysis of Quiescent HIV Infection Reveals Host Transcriptional Profiles that Regulate Proviral Latency.Cell Rep 25, no. 1 (October 2, 2018): 107-117.e3. https://doi.org/10.1016/j.celrep.2018.09.020.
Bradley T, Ferrari G, Haynes BF, Margolis DM, Browne EP. Single-Cell Analysis of Quiescent HIV Infection Reveals Host Transcriptional Profiles that Regulate Proviral Latency. Cell Rep. 2018 Oct 2;25(1):107-117.e3.
Bradley, Todd, et al. “Single-Cell Analysis of Quiescent HIV Infection Reveals Host Transcriptional Profiles that Regulate Proviral Latency.Cell Rep, vol. 25, no. 1, Oct. 2018, pp. 107-117.e3. Pubmed, doi:10.1016/j.celrep.2018.09.020.
Bradley T, Ferrari G, Haynes BF, Margolis DM, Browne EP. Single-Cell Analysis of Quiescent HIV Infection Reveals Host Transcriptional Profiles that Regulate Proviral Latency. Cell Rep. 2018 Oct 2;25(1):107-117.e3.
Journal cover image

Published In

Cell Rep

DOI

EISSN

2211-1247

Publication Date

October 2, 2018

Volume

25

Issue

1

Start / End Page

107 / 117.e3

Location

United States

Related Subject Headings

  • Virus Latency
  • Transcription, Genetic
  • Single-Cell Analysis
  • RNA, Viral
  • Proviruses
  • Jurkat Cells
  • Humans
  • Host Microbial Interactions
  • HIV Infections
  • HIV