Resolvin D1 treatment on goblet cell mucin and immune responses in the chronic allergic eye disease (AED) model.

Published

Journal Article

Severe, chronic eye allergy is an understudied, vision-threatening condition. Treatments remain limited. We used a mouse model of severe allergic eye disease (AED) to determine whether topical application of the pro-resolution mediator Resolvin D1 (RvD1) terminates the response. AED was induced by injection of ovalbumin (OVA) followed by topical challenge of OVA daily. RvD1 was applied topically prior to OVA. Clinical symptoms were scored. Eye washes were assayed for MUC5AC. After 7 days, eyes were removed and the number of goblet cells, T helper cell responses and presence of immune cells in draining lymph nodes and conjunctiva determined. Topical RvD1 treatment significantly reduced symptoms of AED. RvD1 did not alter the systemic type 2 immune response in the lymph nodes. AED increased the total amount of goblet cell mucin secretion, but not the number of goblet cells. RvD1 prevented this increase, but did not alter goblet cell number. Absolute numbers of CD4 + T cells, total CD11b + myeloid cells, eosinophils, neutrophils, and monocytes, but not macrophages increased in AED versus RvD1-treated mice. We conclude that topical application of RvD1 reduced the ocular allergic response by local actions in conjunctival immune response and a decrease in goblet cell mucin secretion.

Full Text

Duke Authors

Cited Authors

  • Saban, DR; Hodges, RR; Mathew, R; Reyes, NJ; Yu, C; Kaye, R; Swift, W; Botten, N; Serhan, CN; Dartt, DA

Published Date

  • January 2019

Published In

Volume / Issue

  • 12 / 1

Start / End Page

  • 145 - 153

PubMed ID

  • 30279513

Pubmed Central ID

  • 30279513

Electronic International Standard Serial Number (EISSN)

  • 1935-3456

International Standard Serial Number (ISSN)

  • 1933-0219

Digital Object Identifier (DOI)

  • 10.1038/s41385-018-0089-1

Language

  • eng