Ferric Chloride-induced Canine Carotid Artery Thrombosis: A Large Animal Model of Vascular Injury.

Journal Article (Journal Article)

Occlusive arterial thrombosis leading to cerebral ischemic stroke and myocardial infarction contributes to ~13 million deaths every year globally. Here, we have translated a vascular injury model from a small animal into a large animal (canine), with slight modifications that can be used for pre-clinical screening of prophylactic and thrombolytic agents. In addition to the surgical methods, the modified protocol describes the step-by-step methods to assess carotid artery canalization by angiography, detailed instructions to process both the brain and carotid artery for histological analysis to verify carotid canalization and cerebral hemorrhage, and specific parameters to complete an assessment of downstream thromboembolic events by utilizing magnetic resonance imaging (MRI). In addition, specific procedural changes from the previously well-established small animal model necessary to translate into a large animal (canine) vascular injury are discussed.

Full Text

Duke Authors

Cited Authors

  • Huttinger, AL; Wheeler, DG; Gnyawali, S; Dornbos, D; Layzer, JM; Venetos, N; Talentino, S; Musgrave, NJ; Jones, C; Bratton, C; Joseph, ME; Sen, C; Sullenger, BA; Nimjee, SM

Published Date

  • September 7, 2018

Published In

PubMed ID

  • 30247470

Pubmed Central ID

  • PMC6235140

Electronic International Standard Serial Number (EISSN)

  • 1940-087X

Digital Object Identifier (DOI)

  • 10.3791/57981


  • eng

Conference Location

  • United States