Simultaneous quantification of vinblastine and desacetylvinblastine concentrations in canine plasma and urine samples using LC-APCI-MS/MS.

Published

Journal Article

A highly sensitive and specific liquid chromatography-atmospheric pressure chemical ionization-mass spectrometry (LC/APCI-MS/MS) method has been developed and validated for simultaneous quantification of vinblastine and its metabolite, desacetylvinblastine, in canine plasma and urine samples. Plasma and urine samples were processed by a solid phase extraction procedure. The optimal chromatographic behavior of these analytes was achieved on pentafluorophenyl (PFP) propyl analytical column (5μm, 50×2.1mm) under isocratic elution of 0.75mL/min with a mobile phase of 5mM ammonium acetate and methanol. The samples were analyzed in positive ion, multiple reaction monitoring mode. The calibration curves were linear over 0.125-2ng/mL (lower calibration curve); 2-100ng/mL (higher calibration curve) and 0.125-5ng/mL for vinblastine and desacetylvinblastine in plasma, and over 1-2000ng/mL and 0.5-100ng/mL for vinblastine and desacetylvinblastine in urine samples, respectively. The limits of quantitation of vinblastine and desacetylvinblastine were 0.125ng/mL in both matrices. The intra and interday accuracy was above 89% and precision below 8.6% for both analytes in both matrices. The developed method was successfully applied to ongoing in vivo vinblastine pharmacokinetic studies in dogs.

Full Text

Duke Authors

Cited Authors

  • Achanta, S; Ngo, M; Veitenheimer, A; Maxwell, LK; Wagner, JR

Published Date

  • January 15, 2013

Published In

Volume / Issue

  • 913-914 /

Start / End Page

  • 147 - 154

PubMed ID

  • 23314352

Pubmed Central ID

  • 23314352

Electronic International Standard Serial Number (EISSN)

  • 1873-376X

Digital Object Identifier (DOI)

  • 10.1016/j.jchromb.2012.11.012

Language

  • eng

Conference Location

  • Netherlands