Tetracycline treatment targeting Wolbachia affects expression of an array of proteins in Brugia malayi parasite.

Published

Journal Article

Wolbachia is an intracellular endosymbiont of Brugia malayi parasite whose presence is essential for the survival of the parasite. Treatment of B. malayi-infected jirds with tetracycline eliminates Wolbachia, which affects parasite survival and fitness. In the present study we have tried to identify parasite proteins that are affected when Wolbachia is targeted by tetracycline. For this Wolbachia depleted parasites (B. malayi) were obtained by tetracycline treatment of infected Mongolian jirds (Meriones unguiculatus) and their protein profile after 2-DE separation was compared with that of untreated parasites harboring Wolbachia. Approximately 100 protein spots could be visualized followed by CBB staining of 2-D gel and included for comparative analysis. Of these, 54 showed differential expressions, while two new protein spots emerged (of 90.3 and 64.4 kDa). These proteins were subjected to further analysis by MALDI-TOF for their identification using Brugia coding sequence database composed of both genomic and EST sequences. Our study unravels two crucial findings: (i) the parasite or Wolbachia proteins, which disappeared/down-regulated appear be essential for parasite survival and may be used as drug targets and (ii) tetracycline treatment interferes with the regulatory machinery vital for parasites cellular integrity and defense and thus could possibly be a molecular mechanism for the killing of filarial parasite. This is the first proteomic study substantiating the wolbachial genome integrity with its nematode host and providing functional genomic data of human lymphatic filarial parasite B. malayi.

Full Text

Duke Authors

Cited Authors

  • Dangi, A; Vedi, S; Nag, JK; Paithankar, S; Singh, MP; Kar, SK; Dube, A; Misra-Bhattacharya, S

Published Date

  • September 2009

Published In

Volume / Issue

  • 9 / 17

Start / End Page

  • 4192 - 4208

PubMed ID

  • 19722191

Pubmed Central ID

  • 19722191

Electronic International Standard Serial Number (EISSN)

  • 1615-9861

Digital Object Identifier (DOI)

  • 10.1002/pmic.200800324

Language

  • eng

Conference Location

  • Germany