Skip to main content
Journal cover image

Over-pressure suppresses ultrasonic-induced drug uptake.

Publication ,  Journal Article
Stringham, SB; Viskovska, MA; Richardson, ES; Ohmine, S; Husseini, GA; Murray, BK; Pitt, WG
Published in: Ultrasound in medicine & biology
March 2009

Ultrasound (US) is used to enhance and target delivery of drugs and genes to cancer tissues. The present study further examines the role of acoustic cavitation in US-induced permeabilization of cell membranes and subsequent drug or gene uptake by the cell. Rat colon cancer cells were exposed to ultrasound at various static pressures to examine the hypothesis that oscillating bubbles, also known as cavitating bubbles, permeabilize cells. Increasing pressure suppresses bubble cavitation activity; thus, if applied pressure were to reduce drug uptake, cell permeabilization would be strongly linked to bubble cavitation activity. Cells were exposed to 476 kHz pulsed ultrasound at average intensities of 2.75 W/cm(2) and 5.5 W/cm(2) at various pressures and times in an isothermal chamber. Cell fractions with reversible membrane damage (calcein uptake) and irreversible damage (propidium iodide uptake) were analyzed by flow cytometry. Pressurization to 3 atm nearly eliminated the biological effect of US in promoting calcein uptake. Data also showed a linear increase in membrane permeability with respect to insonation time and intensity. This research shows that US-mediated cell membrane permeability is likely linked to cavitation bubble activity.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Ultrasound in medicine & biology

DOI

EISSN

1879-291X

ISSN

0301-5629

Publication Date

March 2009

Volume

35

Issue

3

Start / End Page

409 / 415

Related Subject Headings

  • Tumor Cells, Cultured
  • Sonication
  • Rats
  • Pressure
  • Microbubbles
  • Indicators and Reagents
  • Hydrostatic Pressure
  • Fluoresceins
  • Flow Cytometry
  • Drug Delivery Systems
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Stringham, S. B., Viskovska, M. A., Richardson, E. S., Ohmine, S., Husseini, G. A., Murray, B. K., & Pitt, W. G. (2009). Over-pressure suppresses ultrasonic-induced drug uptake. Ultrasound in Medicine & Biology, 35(3), 409–415. https://doi.org/10.1016/j.ultrasmedbio.2008.09.004
Stringham, S Briant, Maria A. Viskovska, Eric S. Richardson, Seiga Ohmine, Ghaleb A. Husseini, Byron K. Murray, and William G. Pitt. “Over-pressure suppresses ultrasonic-induced drug uptake.Ultrasound in Medicine & Biology 35, no. 3 (March 2009): 409–15. https://doi.org/10.1016/j.ultrasmedbio.2008.09.004.
Stringham SB, Viskovska MA, Richardson ES, Ohmine S, Husseini GA, Murray BK, et al. Over-pressure suppresses ultrasonic-induced drug uptake. Ultrasound in medicine & biology. 2009 Mar;35(3):409–15.
Stringham, S. Briant, et al. “Over-pressure suppresses ultrasonic-induced drug uptake.Ultrasound in Medicine & Biology, vol. 35, no. 3, Mar. 2009, pp. 409–15. Epmc, doi:10.1016/j.ultrasmedbio.2008.09.004.
Stringham SB, Viskovska MA, Richardson ES, Ohmine S, Husseini GA, Murray BK, Pitt WG. Over-pressure suppresses ultrasonic-induced drug uptake. Ultrasound in medicine & biology. 2009 Mar;35(3):409–415.
Journal cover image

Published In

Ultrasound in medicine & biology

DOI

EISSN

1879-291X

ISSN

0301-5629

Publication Date

March 2009

Volume

35

Issue

3

Start / End Page

409 / 415

Related Subject Headings

  • Tumor Cells, Cultured
  • Sonication
  • Rats
  • Pressure
  • Microbubbles
  • Indicators and Reagents
  • Hydrostatic Pressure
  • Fluoresceins
  • Flow Cytometry
  • Drug Delivery Systems