High-speed widefield photoacoustic microscopy of small-animal hemodynamics.

Published online

Journal Article

Optical-resolution photoacoustic microscopy (OR-PAM) has become a popular tool in small-animal hemodynamic studies. However, previous OR-PAM techniques variously lacked a high imaging speed and/or a large field of view, impeding the study of highly dynamic physiologic and pathophysiologic processes over a large region of interest. Here we report a high-speed OR-PAM system with an ultra-wide field of view, enabled by an innovative water-immersible hexagon-mirror scanner. By driving the hexagon-mirror scanner with a high-precision DC motor, the new OR-PAM has achieved a cross-sectional frame rate of 900 Hz over a 12-mm scanning range, which is 3900 times faster than our previous motor-scanner-based system and 10 times faster than the MEMS-scanner-based system. Using this hexagon-scanner-based OR-PAM system, we have imaged epinephrine-induced vasoconstriction in the whole mouse ear and vascular reperfusion after ischemic stroke in the mouse cortex in vivo, with a high spatial resolution and high volumetric imaging speed. We expect that the hexagon-scanner-based OR-PAM system will become a powerful tool for small animal imaging where the hemodynamic responses over a large field of view are of interest.

Full Text

Duke Authors

Cited Authors

  • Lan, B; Liu, W; Wang, Y-C; Shi, J; Li, Y; Xu, S; Sheng, H; Zhou, Q; Zou, J; Hoffmann, U; Yang, W; Yao, J

Published Date

  • October 1, 2018

Published In

Volume / Issue

  • 9 / 10

Start / End Page

  • 4689 - 4701

PubMed ID

  • 30319896

Pubmed Central ID

  • 30319896

International Standard Serial Number (ISSN)

  • 2156-7085

Digital Object Identifier (DOI)

  • 10.1364/BOE.9.004689

Language

  • eng

Conference Location

  • United States