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High-speed widefield photoacoustic microscopy of small-animal hemodynamics.

Publication ,  Journal Article
Lan, B; Liu, W; Wang, Y-C; Shi, J; Li, Y; Xu, S; Sheng, H; Zhou, Q; Zou, J; Hoffmann, U; Yang, W; Yao, J
Published in: Biomed Opt Express
October 1, 2018

Optical-resolution photoacoustic microscopy (OR-PAM) has become a popular tool in small-animal hemodynamic studies. However, previous OR-PAM techniques variously lacked a high imaging speed and/or a large field of view, impeding the study of highly dynamic physiologic and pathophysiologic processes over a large region of interest. Here we report a high-speed OR-PAM system with an ultra-wide field of view, enabled by an innovative water-immersible hexagon-mirror scanner. By driving the hexagon-mirror scanner with a high-precision DC motor, the new OR-PAM has achieved a cross-sectional frame rate of 900 Hz over a 12-mm scanning range, which is 3900 times faster than our previous motor-scanner-based system and 10 times faster than the MEMS-scanner-based system. Using this hexagon-scanner-based OR-PAM system, we have imaged epinephrine-induced vasoconstriction in the whole mouse ear and vascular reperfusion after ischemic stroke in the mouse cortex in vivo, with a high spatial resolution and high volumetric imaging speed. We expect that the hexagon-scanner-based OR-PAM system will become a powerful tool for small animal imaging where the hemodynamic responses over a large field of view are of interest.

Duke Scholars

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Published In

Biomed Opt Express

DOI

ISSN

2156-7085

Publication Date

October 1, 2018

Volume

9

Issue

10

Start / End Page

4689 / 4701

Location

United States

Related Subject Headings

  • 5102 Atomic, molecular and optical physics
  • 4003 Biomedical engineering
  • 3212 Ophthalmology and optometry
  • 0912 Materials Engineering
  • 0205 Optical Physics
 

Citation

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Lan, B., Liu, W., Wang, Y.-C., Shi, J., Li, Y., Xu, S., … Yao, J. (2018). High-speed widefield photoacoustic microscopy of small-animal hemodynamics. Biomed Opt Express, 9(10), 4689–4701. https://doi.org/10.1364/BOE.9.004689
Lan, Bangxin, Wei Liu, Ya-Chao Wang, Junhui Shi, Yang Li, Song Xu, Huaxin Sheng, et al. “High-speed widefield photoacoustic microscopy of small-animal hemodynamics.Biomed Opt Express 9, no. 10 (October 1, 2018): 4689–4701. https://doi.org/10.1364/BOE.9.004689.
Lan B, Liu W, Wang Y-C, Shi J, Li Y, Xu S, et al. High-speed widefield photoacoustic microscopy of small-animal hemodynamics. Biomed Opt Express. 2018 Oct 1;9(10):4689–701.
Lan, Bangxin, et al. “High-speed widefield photoacoustic microscopy of small-animal hemodynamics.Biomed Opt Express, vol. 9, no. 10, Oct. 2018, pp. 4689–701. Pubmed, doi:10.1364/BOE.9.004689.
Lan B, Liu W, Wang Y-C, Shi J, Li Y, Xu S, Sheng H, Zhou Q, Zou J, Hoffmann U, Yang W, Yao J. High-speed widefield photoacoustic microscopy of small-animal hemodynamics. Biomed Opt Express. 2018 Oct 1;9(10):4689–4701.
Journal cover image

Published In

Biomed Opt Express

DOI

ISSN

2156-7085

Publication Date

October 1, 2018

Volume

9

Issue

10

Start / End Page

4689 / 4701

Location

United States

Related Subject Headings

  • 5102 Atomic, molecular and optical physics
  • 4003 Biomedical engineering
  • 3212 Ophthalmology and optometry
  • 0912 Materials Engineering
  • 0205 Optical Physics