Incorporation of macrophages into engineered skeletal muscle enables enhanced muscle regeneration.

Published

Journal Article

Adult skeletal muscle has a robust capacity for self-repair, owing to synergies between muscle satellite cells and the immune system. In vitro models of muscle self-repair would facilitate the basic understanding of muscle regeneration and the screening of therapies for muscle disease. Here, we show that the incorporation of macrophages into muscle tissues engineered from adult-rat myogenic cells enables near-complete structural and functional repair after cardiotoxic injury in vitro. First, we show that-in contrast with injured neonatal-derived engineered muscle-adult-derived engineered muscle fails to properly self-repair after injury, even when treated with pro-regenerative cytokines. We then show that rat bone-marrow-derived macrophages or human blood-derived macrophages resident within the in vitro engineered tissues stimulate muscle satellite cell-mediated myogenesis while significantly limiting myofibre apoptosis and degeneration. Moreover, bone-marrow-derived macrophages within engineered tissues implanted in a mouse dorsal window-chamber model augmented blood vessel ingrowth, cell survival, muscle regeneration and contractile function.

Full Text

Duke Authors

Cited Authors

  • Juhas, M; Abutaleb, N; Wang, JT; Ye, J; Shaikh, Z; Sriworarat, C; Qian, Y; Bursac, N

Published Date

  • December 2018

Published In

Volume / Issue

  • 2 / 12

Start / End Page

  • 942 - 954

PubMed ID

  • 30581652

Pubmed Central ID

  • 30581652

Electronic International Standard Serial Number (EISSN)

  • 2157-846X

International Standard Serial Number (ISSN)

  • 2157-846X

Digital Object Identifier (DOI)

  • 10.1038/s41551-018-0290-2

Language

  • eng