Hyperexpression of Foxp3 and IDO during acute rejection of islet allografts.
Published
Journal Article
BACKGROUND: We investigated the hypothesis that Foxp3+ cells are an integral component of antiallograft immunity but are dominated by pathogenic effectors. METHODS: Wild-type H-2b C57BL/6 (B6) mice or B6 mice with a targeted disruption of c-Rel gene (c-Rel-/-) were used as recipients of islet grafts from allogeneic DBA/2 (H-2d) mice or syngeneic B6 mice. We developed kinetic quantitative polymerase chain reaction assays and measured intragraft expression of mRNA for Foxp3, IDO, cytolytic molecules, proinflammatory cytokines, and chemokines/receptors. RESULTS: Intraislet levels of mRNA for Foxp3, IDO, CD3, CD25, tumor necrosis factor-alpha, RANTES, IP-10, and CXCR3 were highest in DBA/2 islet allografts from WT B6 recipients compared to DBA/2 islet allografts from c-Rel-/- B6 recipients or syngeneic B6 islet grafts from WT B6 mice. The ratio of granzyme B or IFN-gamma to Foxp3 was higher with the DBA/2 islet allografts from the WT B6 recipients compared to DBA/2 islet allografts from c-Rel-/- B6 recipients or B6 islet grafts from WT B6 recipients. CONCLUSIONS: Foxp3+ cells are an integral component of acute rejection of allografts but may be dominated by pathogenic effectors.
Full Text
Duke Authors
Cited Authors
- Yang, H; Ding, R; Sharma, VK; Hilaire, FS; Lagman, M; Li, B; Thomas, DA; Luo, X; Song, P; Stauffer, C; August, P; Suthanthiran, M
Published Date
- June 27, 2007
Published In
Volume / Issue
- 83 / 12
Start / End Page
- 1643 - 1647
PubMed ID
- 17589350
Pubmed Central ID
- 17589350
International Standard Serial Number (ISSN)
- 0041-1337
Digital Object Identifier (DOI)
- 10.1097/01.tp.0000263991.74052.46
Language
- eng
Conference Location
- United States