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A flow cytometric crossmatch test using endothelial precursor cells isolated from peripheral blood.

Publication ,  Journal Article
Jackson, AM; Lucas, DP; Badders, JL
Published in: Methods Mol Biol
2013

Flow cytometric crossmatch tests provide a donor-specific, cell based method for the detection of alloreactive antibodies in the sera of transplant patients. Conventional crossmatch tests used in solid organ transplantation utilize lymphocytes as target cells to detect the presence of alloreactive HLA antibodies. Isolation of endothelial precursor cells (EPCs) from peripheral blood now allows testing for antibodies reactive with non-HLA endothelial cell antigens.

Duke Scholars

Published In

Methods Mol Biol

DOI

EISSN

1940-6029

Publication Date

2013

Volume

1034

Start / End Page

319 / 329

Location

United States

Related Subject Headings

  • Transplantation, Homologous
  • Transplantation
  • Tissue Donors
  • Molecular Biology
  • Lymphocytes
  • Humans
  • Histocompatibility Testing
  • HLA Antigens
  • Graft Rejection
  • Flow Cytometry
 

Citation

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ICMJE
MLA
NLM
Jackson, A. M., Lucas, D. P., & Badders, J. L. (2013). A flow cytometric crossmatch test using endothelial precursor cells isolated from peripheral blood. Methods Mol Biol, 1034, 319–329. https://doi.org/10.1007/978-1-62703-493-7_18
Jackson, Annette M., Donna P. Lucas, and Jessica L. Badders. “A flow cytometric crossmatch test using endothelial precursor cells isolated from peripheral blood.Methods Mol Biol 1034 (2013): 319–29. https://doi.org/10.1007/978-1-62703-493-7_18.
Jackson AM, Lucas DP, Badders JL. A flow cytometric crossmatch test using endothelial precursor cells isolated from peripheral blood. Methods Mol Biol. 2013;1034:319–29.
Jackson, Annette M., et al. “A flow cytometric crossmatch test using endothelial precursor cells isolated from peripheral blood.Methods Mol Biol, vol. 1034, 2013, pp. 319–29. Pubmed, doi:10.1007/978-1-62703-493-7_18.
Jackson AM, Lucas DP, Badders JL. A flow cytometric crossmatch test using endothelial precursor cells isolated from peripheral blood. Methods Mol Biol. 2013;1034:319–329.

Published In

Methods Mol Biol

DOI

EISSN

1940-6029

Publication Date

2013

Volume

1034

Start / End Page

319 / 329

Location

United States

Related Subject Headings

  • Transplantation, Homologous
  • Transplantation
  • Tissue Donors
  • Molecular Biology
  • Lymphocytes
  • Humans
  • Histocompatibility Testing
  • HLA Antigens
  • Graft Rejection
  • Flow Cytometry