Mature induced-pluripotent-stem-cell-derived human podocytes reconstitute kidney glomerular-capillary-wall function on a chip.

Published

Journal Article

An in vitro model of the human kidney glomerulus - the major site of blood filtration - could facilitate drug discovery and illuminate kidney-disease mechanisms. Microfluidic organ-on-a-chip technology has been used to model the human proximal tubule, yet a kidney-glomerulus-on-a-chip has not been possible because of the lack of functional human podocytes - the cells that regulate selective permeability in the glomerulus. Here, we demonstrate an efficient (> 90%) and chemically defined method for directing the differentiation of human induced pluripotent stem (hiPS) cells into podocytes that express markers of the mature phenotype (nephrin+, WT1+, podocin+, Pax2-) and that exhibit primary and secondary foot processes. We also show that the hiPS-cell-derived podocytes produce glomerular basement-membrane collagen and recapitulate the natural tissue/tissue interface of the glomerulus, as well as the differential clearance of albumin and inulin, when co-cultured with human glomerular endothelial cells in an organ-on-a-chip microfluidic device. The glomerulus-on-a-chip also mimics adriamycin-induced albuminuria and podocyte injury. This in vitro model of human glomerular function with mature human podocytes may facilitate drug development and personalized-medicine applications.

Full Text

Duke Authors

Cited Authors

  • Musah, S; Mammoto, A; Ferrante, TC; Jeanty, SSF; Hirano-Kobayashi, M; Mammoto, T; Roberts, K; Chung, S; Novak, R; Ingram, M; Fatanat-Didar, T; Koshy, S; Weaver, JC; Church, GM; Ingber, DE

Published Date

  • January 2017

Published In

Volume / Issue

  • 1 /

PubMed ID

  • 29038743

Pubmed Central ID

  • 29038743

Electronic International Standard Serial Number (EISSN)

  • 2157-846X

International Standard Serial Number (ISSN)

  • 2157-846X

Digital Object Identifier (DOI)

  • 10.1038/s41551-017-0069

Language

  • eng