Vapor detection and discrimination with a panel of odorant receptors.

Journal Article (Journal Article)

Olfactory systems have evolved the extraordinary capability to detect and discriminate volatile odorous molecules (odorants) in the environment. Fundamentally, this process relies on the interaction of odorants and their cognate olfactory receptors (ORs) encoded in the genome. Here, we conducted a cell-based screen using over 800 mouse ORs against seven odorants, resulting in the identification of a set of high-affinity and/or broadly-tuned ORs. We then test whether heterologously expressed ORs respond to odors presented in vapor phase by individually expressing 31 ORs to measure cAMP responses against vapor phase odor stimulation. Comparison of response profiles demonstrates this platform is capable of discriminating between structural analogs. Lastly, co-expression of carboxyl esterase Ces1d expressed in olfactory mucosa resulted in marked changes in activation of specific odorant-OR combinations. Altogether, these results establish a cell-based volatile odor detection and discrimination platform and form the basis for an OR-based volatile odor sensor.

Full Text

Duke Authors

Cited Authors

  • Kida, H; Fukutani, Y; Mainland, JD; de March, CA; Vihani, A; Li, YR; Chi, Q; Toyama, A; Liu, L; Kameda, M; Yohda, M; Matsunami, H

Published Date

  • November 1, 2018

Published In

Volume / Issue

  • 9 / 1

Start / End Page

  • 4556 -

PubMed ID

  • 30385742

Pubmed Central ID

  • PMC6212438

Electronic International Standard Serial Number (EISSN)

  • 2041-1723

Digital Object Identifier (DOI)

  • 10.1038/s41467-018-06806-w

Language

  • eng

Conference Location

  • England