Cell-cycle control of cell polarity in yeast.
Journal Article (Journal Article)
In many cells, morphogenetic events are coordinated with the cell cycle by cyclin-dependent kinases (CDKs). For example, many mammalian cells display extended morphologies during interphase but round up into more spherical shapes during mitosis (high CDK activity) and constrict a furrow during cytokinesis (low CDK activity). In the budding yeast Saccharomyces cerevisiae, bud formation reproducibly initiates near the G1/S transition and requires activation of CDKs at a point called "start" in G1. Previous work suggested that CDKs acted by controlling the ability of cells to polarize Cdc42, a conserved Rho-family GTPase that regulates cell polarity and the actin cytoskeleton in many systems. However, we report that yeast daughter cells can polarize Cdc42 before CDK activation at start. This polarization operates via a positive feedback loop mediated by the Cdc42 effector Ste20. We further identify a major and novel locus of CDK action downstream of Cdc42 polarization, affecting the ability of several other Cdc42 effectors to localize to the polarity site.
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Duke Authors
Cited Authors
- Moran, KD; Kang, H; Araujo, AV; Zyla, TR; Saito, K; Tsygankov, D; Lew, DJ
Published Date
- January 7, 2019
Published In
Volume / Issue
- 218 / 1
Start / End Page
- 171 - 189
PubMed ID
- 30459262
Pubmed Central ID
- PMC6314536
Electronic International Standard Serial Number (EISSN)
- 1540-8140
Digital Object Identifier (DOI)
- 10.1083/jcb.201806196
Language
- eng
Conference Location
- United States