Effect of gastric fluid aspiration on the lung microbiota of laboratory rats.

Published

Journal Article

AIM OF THE STUDY:The pulmonary microbiota is important for both normal homeostasis and the progression of disease, and may be affected by aspiration of gastric fluid. The aim of this study was to investigate changes in the lung microbiota induced by aspiration of gastric fluid in a laboratory rat model. MATERIAL AND METHODS:Using the intratracheal application method, male rats received aspiration with 0.9% normal saline (n = 11); gastric fluid (n = 24) or sterilized (gamma-irradiated) gastric fluid (n = 12) once-weekly for four weeks. On the fifth week, the animals were sacrificed, and the microbiota of the lung was assessed by 16S ribosomal RNA gene sequencing. RESULTS:Lungs without aspiration and lungs after aspiration with normal saline had similar microbial compositions, dominated by bacteria of the genera Serratia, Ralstonia and Brucella. Evaluation of the microbiota following aspiration of gastric fluid revealed a much different profile that was dominated by bacteria from the genera Romboutsia and Turicibacter and largely independent of sterilization of the gastric fluid. CONCLUSION:In a laboratory rat model, aspiration with gastric fluid caused a substantial shift of the lung microbiota that could be characterized as a shift from Proteobacteria towards Firmicutes, possibly of enteric origin. Bacteria contained in the gastric fluid are not apparently responsible for this change.

Full Text

Duke Authors

Cited Authors

  • Finn, SMB; Scheuermann, U; Holzknecht, ZE; Parker, W; Granek, JA; Lin, SS; McKenney, EA; Barbas, AS

Published Date

  • May 2018

Published In

Volume / Issue

  • 44 / 4-5

Start / End Page

  • 201 - 210

PubMed ID

  • 30465452

Pubmed Central ID

  • 30465452

Electronic International Standard Serial Number (EISSN)

  • 1521-0499

International Standard Serial Number (ISSN)

  • 0190-2148

Digital Object Identifier (DOI)

  • 10.1080/01902148.2018.1482976

Language

  • eng