A Wor1-Like Transcription Factor Is Essential for Virulence of Cryptococcus neoformans.

Published online

Journal Article

Gti1/Pac2 transcription factors occur exclusively in fungi and their roles vary according to species, including regulating morphological transition and virulence, mating and secondary metabolism. Many of these functions are important for fungal pathogenesis. We therefore hypothesized that one of the two proteins of this family in Cryptococcus neoformans, a major pathogen of humans, would also control virulence-associated cellular processes. Elimination of this protein in C. neoformans results in reduced polysaccharide capsule expression and defective cytokinesis and growth at 37°C. The mutant loses virulence in a mouse model of cryptococcal infection and retains only partial virulence in the Galleria mellonella alternative model at 30°C. We performed RNA-Seq experiments on the mutant and found abolished transcription of genes that, in combination, are known to account for all the observed phenotypes. The protein has been named Required for cytokinesis and virulence 1 (Rcv1).

Full Text

Duke Authors

Cited Authors

  • Paes, HC; Derengowski, LDS; Peconick, LDF; Albuquerque, P; Pappas, GJ; Nicola, AM; Silva, FBA; Vallim, MA; Alspaugh, JA; Felipe, MSS; Fernandes, L

Published Date

  • 2018

Published In

Volume / Issue

  • 8 /

Start / End Page

  • 369 -

PubMed ID

  • 30483479

Pubmed Central ID

  • 30483479

Electronic International Standard Serial Number (EISSN)

  • 2235-2988

Digital Object Identifier (DOI)

  • 10.3389/fcimb.2018.00369

Language

  • eng

Conference Location

  • Switzerland