A One-Step Staining Protocol for In-Gel Fluorescent Visualization of Proteins.

Book Section

Native polyacrylamide gel electrophoresis (PAGE) and sodium dodecyl sulfate (SDS)-PAGE are among the most frequently applied techniques in protein analysis. Here we describe a fast one-step method for fluorescent visualization of proteins. Following PAGE, gels are soaked in solution of potassium ferricyanide (100 mM) in 1 M NaOH, and are kept in the dark for 30 min. Gels are then transferred to water and scanned. The sensitivity of the method is comparable with standard Coomassie Brilliant Blue staining.

Full Text

Duke Authors

Cited Authors

  • Bogdanović Pristov, J; Spasojević, I

Published Date

  • 2018

Volume / Issue

  • 1853 /

Start / End Page

  • 149 - 158

PubMed ID

  • 30097940

Digital Object Identifier (DOI)

  • 10.1007/978-1-4939-8745-0_18