Spore persistence and likelihood of aeroallergenicity of entomopathogenic fungi used for mosquito control.


Journal Article

Entomopathogenic fungi, such as Metarhizium anisopliae and Beauveria bassiana, are being researched as alternatives to chemical adulticides to control mosquito vectors of malaria and dengue. Two cited concerns of fungal control include conidial viability and risks fungal entomopathogens pose to human health. We measured spore viability of 10 fungal isolates over 26 weeks and found a range of persistence. Three B. bassiana isolates maintained > or = 50% viability 14 weeks after application. No M. anisopliae isolate persisted longer than 1 week. To help assess risk of conidia as potential human allergens, we measured airborne conidia in enclosed environments after simulated biopesticide treatment of M. anisopliae. Conidia were detectable immediately after treatment, with concentrations of ~7000/m(3), decreasing over 48 hours to 500 conidia/m(3). At most, Metarhizium conidia comprised 2% of total visible particulate matter, falling to 0.1% by 2 days. The implications for viability of biological control of adult mosquitoes are discussed.

Full Text

Cited Authors

  • Darbro, JM; Thomas, MB

Published Date

  • June 2009

Published In

Volume / Issue

  • 80 / 6

Start / End Page

  • 992 - 997

PubMed ID

  • 19478264

Pubmed Central ID

  • 19478264

Electronic International Standard Serial Number (EISSN)

  • 1476-1645

International Standard Serial Number (ISSN)

  • 0002-9637

Digital Object Identifier (DOI)

  • 10.4269/ajtmh.2009.80.992


  • eng