Macrophage cells secrete factors including LRP1 that orchestrate the rejuvenation of bone repair in mice.

Published online

Journal Article

The pace of repair declines with age and, while exposure to a young circulation can rejuvenate fracture repair, the cell types and factors responsible for rejuvenation are unknown. Here we report that young macrophage cells produce factors that promote osteoblast differentiation of old bone marrow stromal cells. Heterochronic parabiosis exploiting young mice in which macrophages can be depleted and fractionated bone marrow transplantation experiments show that young macrophages rejuvenate fracture repair, and old macrophage cells slow healing in young mice. Proteomic analysis of the secretomes identify differential proteins secreted between old and young macrophages, such as low-density lipoprotein receptor-related protein 1 (Lrp1). Lrp1 is produced by young cells, and depleting Lrp1 abrogates the ability to rejuvenate fracture repair, while treating old mice with recombinant Lrp1 improves fracture healing. Macrophages and proteins they secrete orchestrate the fracture repair process, and young cells produce proteins that rejuvenate fracture repair in mice.

Full Text

Duke Authors

Cited Authors

  • Vi, L; Baht, GS; Soderblom, EJ; Whetstone, H; Wei, Q; Furman, B; Puviindran, V; Nadesan, P; Foster, M; Poon, R; White, JP; Yahara, Y; Ng, A; Barrientos, T; Grynpas, M; Mosely, MA; Alman, BA

Published Date

  • December 5, 2018

Published In

Volume / Issue

  • 9 / 1

Start / End Page

  • 5191 -

PubMed ID

  • 30518764

Pubmed Central ID

  • 30518764

Electronic International Standard Serial Number (EISSN)

  • 2041-1723

Digital Object Identifier (DOI)

  • 10.1038/s41467-018-07666-0

Language

  • eng

Conference Location

  • England