Ankyrin-G regulates forebrain connectivity and network synchronization via interaction with GABARAP.
Journal Article (Journal Article)
GABAergic circuits are critical for the synchronization and higher order function of brain networks. Defects in this circuitry are linked to neuropsychiatric diseases, including bipolar disorder, schizophrenia, and autism. Work in cultured neurons has shown that ankyrin-G plays a key role in the regulation of GABAergic synapses on the axon initial segment and somatodendritic domain of pyramidal neurons, where it interacts directly with the GABAA receptor-associated protein (GABARAP) to stabilize cell surface GABAA receptors. Here, we generated a knock-in mouse model expressing a mutation that abolishes the ankyrin-G/GABARAP interaction (Ank3 W1989R) to understand how ankyrin-G and GABARAP regulate GABAergic circuitry in vivo. We found that Ank3 W1989R mice exhibit a striking reduction in forebrain GABAergic synapses resulting in pyramidal cell hyperexcitability and disruptions in network synchronization. In addition, we identified changes in pyramidal cell dendritic spines and axon initial segments consistent with compensation for hyperexcitability. Finally, we identified the ANK3 W1989R variant in a family with bipolar disorder, suggesting a potential role of this variant in disease. Our results highlight the importance of ankyrin-G in regulating forebrain circuitry and provide novel insights into how ANK3 loss-of-function variants may contribute to human disease.
Full Text
Duke Authors
Cited Authors
- Nelson, AD; Caballero-Florán, RN; Rodríguez Díaz, JC; Hull, JM; Yuan, Y; Li, J; Chen, K; Walder, KK; Lopez-Santiago, LF; Bennett, V; McInnis, MG; Isom, LL; Wang, C; Zhang, M; Jones, KS; Jenkins, PM
Published Date
- November 2020
Published In
Volume / Issue
- 25 / 11
Start / End Page
- 2800 - 2817
PubMed ID
- 30504823
Pubmed Central ID
- PMC6542726
Electronic International Standard Serial Number (EISSN)
- 1476-5578
Digital Object Identifier (DOI)
- 10.1038/s41380-018-0308-x
Language
- eng
Conference Location
- England