Ultrastructure of hemoglobin-depleted human erythrocyte resealed ghosts.

Journal Article

Both negative-stain and freeze-fracture electron microscopic techniques revealed that the ultrastructure of resealed white ghosts prepared at high dilution during the hemolysis step is very different from that of resealed ghosts prepared at low or moderate dilution (pink ghosts). The negative-stained resealed white ghosts showed light halo substructures on membrane surfaces and protrusions at the edge of the ghosts. Freeze-fracturing of these ghosts showed that membrane blebbing had occurred and that fragments of the membranes resealed to form small right-side-out vesicles ranging from 0.1 to 0.3 micrometer in diameter.

Full Text

Duke Authors

Cited Authors

  • Ting-Beall, HP; Costello, MJ; Shoemaker, D; Holland, VF

Published Date

  • February 6, 1981

Published In

Volume / Issue

  • 640 / 3

Start / End Page

  • 807 - 811

PubMed ID

  • 7213706

Pubmed Central ID

  • 7213706

International Standard Serial Number (ISSN)

  • 0006-3002

Digital Object Identifier (DOI)

  • 10.1016/0005-2736(81)90111-5

Language

  • eng

Conference Location

  • Netherlands