Cryo-electron microscopy of biological samples.

Conference Paper

A brief summary of current cryo-electron microscopy methods for processing and imaging biological tissues is provided. The main emphasis is given to two preparation procedures: frozen-hydrated samples because of the remarkable success of cryo-electron crystallography in obtaining near atomic resolution of integral membrane proteins, and high-pressure freezing because of the wide applicability for vitrification of large samples of normal and diseased tissues for ultrastructural and immunolabeling analysis. Methods for examining certain samples with a TEM cryo-stage are summarized. This includes an introduction to the relatively new area of cryo-electron tomography, which offers the possibility to observe the three-dimensional structure of subcellular components using only their natural variations in composition to generate contrast.

Full Text

Duke Authors

Cited Authors

  • Costello, MJ

Published Date

  • 2006

Published In

Volume / Issue

  • 30 / 5

Start / End Page

  • 361 - 371

PubMed ID

  • 17090515

Electronic International Standard Serial Number (EISSN)

  • 1521-0758

Digital Object Identifier (DOI)

  • 10.1080/01913120600932735

Conference Location

  • England