Cryo-electron microscopy of biological samples.
Conference Paper
A brief summary of current cryo-electron microscopy methods for processing and imaging biological tissues is provided. The main emphasis is given to two preparation procedures: frozen-hydrated samples because of the remarkable success of cryo-electron crystallography in obtaining near atomic resolution of integral membrane proteins, and high-pressure freezing because of the wide applicability for vitrification of large samples of normal and diseased tissues for ultrastructural and immunolabeling analysis. Methods for examining certain samples with a TEM cryo-stage are summarized. This includes an introduction to the relatively new area of cryo-electron tomography, which offers the possibility to observe the three-dimensional structure of subcellular components using only their natural variations in composition to generate contrast.
Full Text
Duke Authors
Cited Authors
- Costello, MJ
Published Date
- 2006
Published In
Volume / Issue
- 30 / 5
Start / End Page
- 361 - 371
PubMed ID
- 17090515
Electronic International Standard Serial Number (EISSN)
- 1521-0758
Digital Object Identifier (DOI)
- 10.1080/01913120600932735
Conference Location
- England