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Characterization of corneal proteoglycans under vitamin A deficiency.

Publication ,  Journal Article
Twining, SS; Wilson, PM; Hatchell, DL
Published in: Biochim Biophys Acta
August 18, 1989

Comparison of in vivo radiolabeled corneal proteoglycans from vitamin A deficient, pair-fed control and normal rabbits by chromatographic and enzymatic methods, reveals subtle but reproducible changes in the proteoglycans present in the vitamin A deficient corneas relative to those of pair-fed and normal control corneas. Although the total amounts of [3H]leucine and [35S]sulfate incorporated in vivo into the proteoglycans per cornea are similar in the three types of cornea, the proteoglycan affinity for DEAE-Sepharose is different, with more of the vitamin A deficient proteoglycans requiring a higher NaCl concentration for elution. Analysis of in vivo labeled rabbit corneal proteoglycans reveals that in vitamin A deficient animals, relative to pair-fed controls, there is (1) an increase in the proteoglycans digested by chondroitinase AC indicative of decreased epimerization of glucuronic acid to iduronic acid; (2) an increase in the amount of keratan sulfate proteoglycan with high affinity for an anion exchange resin, consistent with a greater negative charge; (3) differences in proteoglycan affinities for octyl-Sepharose, reflecting differences in hydrophobicity; (4) increased susceptibility to proteolysis by trypsin; (5) no significant difference in sulfation.

Duke Scholars

Published In

Biochim Biophys Acta

DOI

ISSN

0006-3002

Publication Date

August 18, 1989

Volume

992

Issue

2

Start / End Page

181 / 190

Location

Netherlands

Related Subject Headings

  • Vitamin A Deficiency
  • Trypsin
  • Rabbits
  • Proteoglycans
  • Male
  • Hydrolysis
  • Glycoside Hydrolases
  • Glycosaminoglycans
  • Eye Proteins
  • Corneal Diseases
 

Citation

APA
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MLA
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Twining, S. S., Wilson, P. M., & Hatchell, D. L. (1989). Characterization of corneal proteoglycans under vitamin A deficiency. Biochim Biophys Acta, 992(2), 181–190. https://doi.org/10.1016/0304-4165(89)90008-1
Twining, S. S., P. M. Wilson, and D. L. Hatchell. “Characterization of corneal proteoglycans under vitamin A deficiency.Biochim Biophys Acta 992, no. 2 (August 18, 1989): 181–90. https://doi.org/10.1016/0304-4165(89)90008-1.
Twining SS, Wilson PM, Hatchell DL. Characterization of corneal proteoglycans under vitamin A deficiency. Biochim Biophys Acta. 1989 Aug 18;992(2):181–90.
Twining, S. S., et al. “Characterization of corneal proteoglycans under vitamin A deficiency.Biochim Biophys Acta, vol. 992, no. 2, Aug. 1989, pp. 181–90. Pubmed, doi:10.1016/0304-4165(89)90008-1.
Twining SS, Wilson PM, Hatchell DL. Characterization of corneal proteoglycans under vitamin A deficiency. Biochim Biophys Acta. 1989 Aug 18;992(2):181–190.

Published In

Biochim Biophys Acta

DOI

ISSN

0006-3002

Publication Date

August 18, 1989

Volume

992

Issue

2

Start / End Page

181 / 190

Location

Netherlands

Related Subject Headings

  • Vitamin A Deficiency
  • Trypsin
  • Rabbits
  • Proteoglycans
  • Male
  • Hydrolysis
  • Glycoside Hydrolases
  • Glycosaminoglycans
  • Eye Proteins
  • Corneal Diseases