Suppression of human corneal epithelial proliferation with breast carcinoma immunotoxin.


Journal Article

We examined the effects of the immunotoxin 260F9 Mab-recombinant ricin A (developed against human breast carcinoma) on proliferating and confluent human corneal epithelium (HCE) cells in vitro. HCE cells derived from explants of discarded human donor corneoscleral rims were established as proliferating and confluent cell cultures, and were exposed continuously for 7 days to immunotoxin. Final cell counts at day 7, and thymidine uptake measured at days 1 and 7 postexposure, showed > 95% suppression of proliferating cells at an immunotoxin concentration of 10 ng/ml, with confluent HCE cells relatively unaffected. This immunotoxin may prove useful in treatment of proliferative ocular epithelial diseases such as epithelial downgrowth or squamous cell carcinoma of the ocular surface.

Full Text

Duke Authors

Cited Authors

  • Fulcher, S; Foulks, GN; Wilkerson, M; Cobo, LM; Houston, LL; Hatchell, D

Published Date

  • September 1, 1993

Published In

Volume / Issue

  • 12 / 5

Start / End Page

  • 391 - 396

PubMed ID

  • 8306659

Pubmed Central ID

  • 8306659

Electronic International Standard Serial Number (EISSN)

  • 1536-4798

International Standard Serial Number (ISSN)

  • 0277-3740


  • eng