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Evaluation of Poly (Carbonate-Urethane) Urea (PCUU) Scaffolds for Urinary Bladder Tissue Engineering.

Publication ,  Journal Article
Sivaraman, S; Amoroso, N; Gu, X; Purves, JT; Hughes, FM; Wagner, WR; Nagatomi, J
Published in: Ann Biomed Eng
March 2019

Although the previous success of bladder tissue engineering demonstrated the feasibility of this technology, most polyester based scaffolds used in previous studies possess inadequate mechanical properties for organs that exhibit large deformation. The present study explored the use of various biodegradable elastomers as scaffolds for bladder tissue engineering and poly (carbonate-urethane) urea (PCUU) scaffolds mimicked urinary bladder mechanics more closely than polyglycerol sebacate-polycaprolactone (PGS-PCL) and poly (ether-urethane) urea (PEUU). The PCUU scaffolds also showed cyto-compatibility as well as increased porosity with increasing stretch indicating its ability to aid in infiltration of smooth muscle cells. Moreover, a bladder outlet obstruction (BOO) rat model was used to test the safety and efficacy of the PCUU scaffolds in treating a voiding dysfunction. Bladder augmentation with PCUU scaffolds led to enhanced survival of the rats and an increase in the bladder capacity and voiding volume over a 3 week period, indicating that the high-pressure bladder symptom common to BOO was alleviated. The histological analysis of the explanted scaffold demonstrated smooth muscle cell and connective tissue infiltration. The knowledge gained in the present study should contribute towards future improvement of bladder tissue engineering technology to ultimately aide in the treatment of bladder disorders.

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Published In

Ann Biomed Eng

DOI

EISSN

1573-9686

Publication Date

March 2019

Volume

47

Issue

3

Start / End Page

891 / 901

Location

United States

Related Subject Headings

  • Urinary Bladder Neck Obstruction
  • Urinary Bladder
  • Tissue Scaffolds
  • Tissue Engineering
  • Rats, Sprague-Dawley
  • Polymers
  • Myocytes, Smooth Muscle
  • Female
  • Cells, Cultured
  • Cell Survival
 

Citation

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Sivaraman, S., Amoroso, N., Gu, X., Purves, J. T., Hughes, F. M., Wagner, W. R., & Nagatomi, J. (2019). Evaluation of Poly (Carbonate-Urethane) Urea (PCUU) Scaffolds for Urinary Bladder Tissue Engineering. Ann Biomed Eng, 47(3), 891–901. https://doi.org/10.1007/s10439-018-02182-0
Sivaraman, Srikanth, Nicholas Amoroso, Xinzhu Gu, J Todd Purves, Francis M. Hughes, William R. Wagner, and Jiro Nagatomi. “Evaluation of Poly (Carbonate-Urethane) Urea (PCUU) Scaffolds for Urinary Bladder Tissue Engineering.Ann Biomed Eng 47, no. 3 (March 2019): 891–901. https://doi.org/10.1007/s10439-018-02182-0.
Sivaraman S, Amoroso N, Gu X, Purves JT, Hughes FM, Wagner WR, et al. Evaluation of Poly (Carbonate-Urethane) Urea (PCUU) Scaffolds for Urinary Bladder Tissue Engineering. Ann Biomed Eng. 2019 Mar;47(3):891–901.
Sivaraman, Srikanth, et al. “Evaluation of Poly (Carbonate-Urethane) Urea (PCUU) Scaffolds for Urinary Bladder Tissue Engineering.Ann Biomed Eng, vol. 47, no. 3, Mar. 2019, pp. 891–901. Pubmed, doi:10.1007/s10439-018-02182-0.
Sivaraman S, Amoroso N, Gu X, Purves JT, Hughes FM, Wagner WR, Nagatomi J. Evaluation of Poly (Carbonate-Urethane) Urea (PCUU) Scaffolds for Urinary Bladder Tissue Engineering. Ann Biomed Eng. 2019 Mar;47(3):891–901.
Journal cover image

Published In

Ann Biomed Eng

DOI

EISSN

1573-9686

Publication Date

March 2019

Volume

47

Issue

3

Start / End Page

891 / 901

Location

United States

Related Subject Headings

  • Urinary Bladder Neck Obstruction
  • Urinary Bladder
  • Tissue Scaffolds
  • Tissue Engineering
  • Rats, Sprague-Dawley
  • Polymers
  • Myocytes, Smooth Muscle
  • Female
  • Cells, Cultured
  • Cell Survival