Methods for transplantation of sea urchin blastomeres.
Publication
, Journal Article
George, AN; McClay, DR
Published in: Methods in cell biology
January 2019
The stereotypic cleavage pattern of sea urchin embryos provides a platform for dissection of early lineage decisions that lead to cell diversification. Cell transplantation provides a useful tool for understanding those decisions. The methods described in this paper provide a guide for how to produce embryonic mosaics in which either one cell is transplanted or an entire tier of cells are transplanted to a host embryo. Although the results of such a cut and paste experiment can be documented in many ways, one of the most useful approaches follows progeny of the transplanted cell as they go through morphogenesis using time-lapse imaging. Methods for mounting and imaging the embryos are provided.
Duke Scholars
Published In
Methods in cell biology
DOI
ISSN
0091-679X
Publication Date
January 2019
Volume
150
Start / End Page
223 / 233
Related Subject Headings
- Sea Urchins
- Morphogenesis
- Embryo, Nonmammalian
- Developmental Biology
- Cell Transplantation
- Blastomeres
- Animals
- 3101 Biochemistry and cell biology
- 0601 Biochemistry and Cell Biology
Citation
APA
Chicago
ICMJE
MLA
NLM
George, A. N., & McClay, D. R. (2019). Methods for transplantation of sea urchin blastomeres. Methods in Cell Biology, 150, 223–233. https://doi.org/10.1016/bs.mcb.2018.11.012
George, Andrew N., and David R. McClay. “Methods for transplantation of sea urchin blastomeres.” Methods in Cell Biology 150 (January 2019): 223–33. https://doi.org/10.1016/bs.mcb.2018.11.012.
George AN, McClay DR. Methods for transplantation of sea urchin blastomeres. Methods in cell biology. 2019 Jan;150:223–33.
George, Andrew N., and David R. McClay. “Methods for transplantation of sea urchin blastomeres.” Methods in Cell Biology, vol. 150, Jan. 2019, pp. 223–33. Epmc, doi:10.1016/bs.mcb.2018.11.012.
George AN, McClay DR. Methods for transplantation of sea urchin blastomeres. Methods in cell biology. 2019 Jan;150:223–233.
Published In
Methods in cell biology
DOI
ISSN
0091-679X
Publication Date
January 2019
Volume
150
Start / End Page
223 / 233
Related Subject Headings
- Sea Urchins
- Morphogenesis
- Embryo, Nonmammalian
- Developmental Biology
- Cell Transplantation
- Blastomeres
- Animals
- 3101 Biochemistry and cell biology
- 0601 Biochemistry and Cell Biology