Methods for transplantation of sea urchin blastomeres.
Journal Article (Review;Journal Article)
The stereotypic cleavage pattern of sea urchin embryos provides a platform for dissection of early lineage decisions that lead to cell diversification. Cell transplantation provides a useful tool for understanding those decisions. The methods described in this paper provide a guide for how to produce embryonic mosaics in which either one cell is transplanted or an entire tier of cells are transplanted to a host embryo. Although the results of such a cut and paste experiment can be documented in many ways, one of the most useful approaches follows progeny of the transplanted cell as they go through morphogenesis using time-lapse imaging. Methods for mounting and imaging the embryos are provided.
Full Text
Duke Authors
Cited Authors
- George, AN; McClay, DR
Published Date
- January 2019
Published In
Volume / Issue
- 150 /
Start / End Page
- 223 - 233
PubMed ID
- 30777177
Pubmed Central ID
- 30777177
International Standard Serial Number (ISSN)
- 0091-679X
Digital Object Identifier (DOI)
- 10.1016/bs.mcb.2018.11.012
Language
- eng