Methods for transplantation of sea urchin blastomeres.

Published

Journal Article (Review)

The stereotypic cleavage pattern of sea urchin embryos provides a platform for dissection of early lineage decisions that lead to cell diversification. Cell transplantation provides a useful tool for understanding those decisions. The methods described in this paper provide a guide for how to produce embryonic mosaics in which either one cell is transplanted or an entire tier of cells are transplanted to a host embryo. Although the results of such a cut and paste experiment can be documented in many ways, one of the most useful approaches follows progeny of the transplanted cell as they go through morphogenesis using time-lapse imaging. Methods for mounting and imaging the embryos are provided.

Full Text

Duke Authors

Cited Authors

  • George, AN; McClay, DR

Published Date

  • January 2019

Published In

Volume / Issue

  • 150 /

Start / End Page

  • 223 - 233

PubMed ID

  • 30777177

Pubmed Central ID

  • 30777177

International Standard Serial Number (ISSN)

  • 0091-679X

Digital Object Identifier (DOI)

  • 10.1016/bs.mcb.2018.11.012

Language

  • eng