Decreased S -Nitrosylation of Tissue Transglutaminase Contributes to Age-Related Increases in Vascular Stiffness

Journal Article (Academic article)

Objective: We sought to determine whether endothelium-dependent NO regulates TG2 activity by S-nitrosylation and whether this contributes to age-related vascular stiffness. Methods and Results: We first demonstrate that NO suppresses activity and increases S-nitrosylation of TG2 in cellular models. Next, we show that nitric oxide synthase (NOS) inhibition leads to increased surface and extracellular matrix–associated TG2. We then demonstrate that endothelium-derived bioactive NO primarily mediates its effects through TG2, using TG2؊/؊ mice chronically treated with the NOS inhibitor L-NGnitroarginine methyl ester (L-NAME). We confirm that TG2 activity is modulated by endothelium-derived bioactive NO in young rat aorta. In aging rat aorta, although TG2 expression remains unaltered, its activity increases and S-nitrosylation decreases. Furthermore, TG2 inhibition decreases vascular stiffness in aging rats. Finally, TG2 activity and matrix crosslinks are augmented with age in human aorta, whereas abundance remains unchanged. Conclusions: Decreased S-nitrosylation of TG2 and increased TG activity lead to enhanced matrix crosslinking and contribute to vascular stiffening in aging. TG2 appears to be the member of the transglutaminase family primarily contributing to this phenotype. Inhibition of TG2 could thus represent a therapeutic target for age-associated vascular stiffness and isolated systolic hypertension. (Circ Res. 2010;107:117-125.)

Full Text

Duke Authors

Cited Authors

  • Santhanam, L; Tuday, EC; Webb, AK; Dowzicky, P; Kim, JH; Oh, YJ; Sikka, G; Kuo, M; Halushka, MK; Macgregor, AM; Dunn, J; Gutbrod, S; Yin, D; Shoukas, A; Nyhan, D; Flavahan, NA; Belkin, AM; Berkowitz, DE

Published Date

  • July 2010

Published In

  • Circulation Research

Volume / Issue

  • 107 /


  • 1

Start / End Page

  • 117 - 125

PubMed ID

  • 20489165

Digital Object Identifier (DOI)

  • 10.1161/CIRCRESAHA.109.215228


  • en