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S-Nitrosation of arginase 1 requires direct interaction with inducible nitric oxide synthase

Publication ,  Journal Article
Dunn, J; Gutbrod, S; Webb, A; Pak, A; Jandu, SK; Bhunia, A; Berkowitz, DE; Santhanam, L
Published in: Molecular and Cellular Biochemistry
September 2011

Arginase constrains endothelial nitric oxide synthase activity by competing for the common substrate, L-Arginine. We have recently shown that inducible nitric oxide synthase (NOS2) Snitrosates and activates arginase 1 (Arg1) leading to age-associated vascular dysfunction. Here, we demonstrate that a direct interaction of Arg1 with NOS2 is necessary for its S-nitrosation. The specific domain of NOS2 that mediates this interaction is identified. Disruption of this interaction in human aortic endothelial cells prevents Arg1 S-nitrosation and activation. Thus, disruption of NOS2–Arg1 interaction may represent a therapeutic strategy to attenuate age related vascular endothelial dysfunction.

Duke Scholars

Published In

Molecular and Cellular Biochemistry

DOI

Publication Date

September 2011

Volume

355

Start / End Page

83 / 89

Related Subject Headings

  • Protein Structure, Tertiary
  • Protein Binding
  • Peptide Fragments
  • Nitrosation
  • Nitric Oxide Synthase Type II
  • Nitrates
  • Mice, Knockout
  • Mice, Inbred C57BL
  • Mice
  • Lipopolysaccharides
 

Citation

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Dunn, J., Gutbrod, S., Webb, A., Pak, A., Jandu, S. K., Bhunia, A., … Santhanam, L. (2011). S-Nitrosation of arginase 1 requires direct interaction with inducible nitric oxide synthase. Molecular and Cellular Biochemistry, 355, 83–89. https://doi.org/10.1007/s11010-011-0841-2
Dunn, Jessilyn, Sarah Gutbrod, Alanah Webb, Alina Pak, Simran K. Jandu, Anil Bhunia, Dan E. Berkowitz, and Lakshmi Santhanam. “S-Nitrosation of arginase 1 requires direct interaction with inducible nitric oxide synthase.” Molecular and Cellular Biochemistry 355 (September 2011): 83–89. https://doi.org/10.1007/s11010-011-0841-2.
Dunn J, Gutbrod S, Webb A, Pak A, Jandu SK, Bhunia A, et al. S-Nitrosation of arginase 1 requires direct interaction with inducible nitric oxide synthase. Molecular and Cellular Biochemistry. 2011 Sep;355:83–9.
Dunn, Jessilyn, et al. “S-Nitrosation of arginase 1 requires direct interaction with inducible nitric oxide synthase.” Molecular and Cellular Biochemistry, vol. 355, Sept. 2011, pp. 83–89. Manual, doi:10.1007/s11010-011-0841-2.
Dunn J, Gutbrod S, Webb A, Pak A, Jandu SK, Bhunia A, Berkowitz DE, Santhanam L. S-Nitrosation of arginase 1 requires direct interaction with inducible nitric oxide synthase. Molecular and Cellular Biochemistry. 2011 Sep;355:83–89.

Published In

Molecular and Cellular Biochemistry

DOI

Publication Date

September 2011

Volume

355

Start / End Page

83 / 89

Related Subject Headings

  • Protein Structure, Tertiary
  • Protein Binding
  • Peptide Fragments
  • Nitrosation
  • Nitric Oxide Synthase Type II
  • Nitrates
  • Mice, Knockout
  • Mice, Inbred C57BL
  • Mice
  • Lipopolysaccharides