S-Nitrosation of arginase 1 requires direct interaction with inducible nitric oxide synthase

Journal Article (Academic article)

Arginase constrains endothelial nitric oxide synthase activity by competing for the common substrate, L-Arginine. We have recently shown that inducible nitric oxide synthase (NOS2) Snitrosates and activates arginase 1 (Arg1) leading to age-associated vascular dysfunction. Here, we demonstrate that a direct interaction of Arg1 with NOS2 is necessary for its S-nitrosation. The specific domain of NOS2 that mediates this interaction is identified. Disruption of this interaction in human aortic endothelial cells prevents Arg1 S-nitrosation and activation. Thus, disruption of NOS2–Arg1 interaction may represent a therapeutic strategy to attenuate age related vascular endothelial dysfunction.

Full Text

Duke Authors

Cited Authors

  • Dunn, J; Gutbrod, S; Webb, A; Pak, A; Jandu, SK; Bhunia, A; Berkowitz, DE; Santhanam, L

Published Date

  • September 2011

Published In

  • Molecular and Cellular Biochemistry

Volume / Issue

  • 355 /

Chapter

  • 1-2

Start / End Page

  • 83 - 89

Digital Object Identifier (DOI)

  • 10.1007/s11010-011-0841-2

Language

  • en