MRGPR-mediated activation of local mast cells clears cutaneous bacterial infection and protects against reinfection.

Published online

Journal Article

Mast cells (MCs) are strategically distributed at barrier sites and prestore various immunocyte-recruiting cytokines, making them ideal targets for selective activation to treat peripheral infections. Here, we report that topical treatment with mastoparan, a peptide MC activator (MCA), enhances clearance of Staphylococcus aureus from infected mouse skins and accelerates healing of dermonecrotic lesions. Mastoparan functions by activating connective tissue MCs (CTMCs) via the MRGPRX2 (Mas-related G protein-coupled receptor member X2) receptor. Peripheral CTMC activation, in turn, enhances recruitment of bacteria-clearing neutrophils and wound-healing CD301b+ dendritic cells. Consistent with MCs playing a master coordinating role, MC activation also augmented migration of various antigen-presenting dendritic cells to draining lymph nodes, leading to stronger protection against a second infection challenge. MCAs therefore orchestrate both the innate and adaptive immune arms, which could potentially be applied to combat peripheral infections by a broad range of pathogens.

Full Text

Duke Authors

Cited Authors

  • Arifuzzaman, M; Mobley, YR; Choi, HW; Bist, P; Salinas, CA; Brown, ZD; Chen, SL; Staats, HF; Abraham, SN

Published Date

  • January 2019

Published In

Volume / Issue

  • 5 / 1

Start / End Page

  • eaav0216 -

PubMed ID

  • 30613778

Pubmed Central ID

  • 30613778

Electronic International Standard Serial Number (EISSN)

  • 2375-2548

Digital Object Identifier (DOI)

  • 10.1126/sciadv.aav0216

Language

  • eng

Conference Location

  • United States