Cryptococcus neoformans Mating and Genetic Crosses.

Journal Article (Journal Article)

The Cryptococcus pathogenic species complex is a group of opportunistic human fungal pathogens that cause cryptococcal meningoencephalitis, an infection associated with unacceptably high mortality rates. The public health relevance of these pathogens has galvanized extensive research over the past several decades and led to characterization of their sexual cycles. This research has allowed several Cryptococcus species to develop into model fungal organisms for both pathogenesis and basic science studies. Many of these studies require observation of the meiotic process and its associated mating structures as well as generation of meiotic progeny with novel phenotypes and genotypes. Herein, we describe how to set up genetic crosses between Cryptococcus strains and observe their mating phenotypes as well as how to recover progeny from these crosses for further analysis. © 2019 by John Wiley & Sons, Inc.

Full Text

Duke Authors

Cited Authors

  • Sun, S; Priest, SJ; Heitman, J

Published Date

  • June 2019

Published In

Volume / Issue

  • 53 / 1

Start / End Page

  • e75 -

PubMed ID

  • 30661293

Pubmed Central ID

  • PMC6586516

Electronic International Standard Serial Number (EISSN)

  • 1934-8533

Digital Object Identifier (DOI)

  • 10.1002/cpmc.75

Language

  • eng

Conference Location

  • United States