Genome-wide analysis of genomic alterations induced by oxidative DNA damage in yeast.

Published

Journal Article

Oxidative DNA damage is a threat to genome stability. Using a genetic system in yeast that allows detection of mitotic recombination, we found that the frequency of crossovers is greatly elevated when cells are treated with hydrogen peroxide (H2O2). Using a combination of microarray analysis and genomic sequencing, we mapped the breakpoints of mitotic recombination events and other chromosome rearrangements at a resolution of about 1 kb. Gene conversions and crossovers were the two most common types of events, but we also observed deletions, duplications, and chromosome aneuploidy. In addition, H2O2-treated cells had elevated rates of point mutations (particularly A to T/T to A and C to G/G to C transversions) and small insertions/deletions (in/dels). In cells that underwent multiple rounds of H2O2 treatments, we identified a genetic alteration that resulted in improved H2O2 tolerance by amplification of the CTT1 gene that encodes cytosolic catalase T. Lastly, we showed that cells grown in the absence of oxygen have reduced levels of recombination. This study provided multiple novel insights into how oxidative stress affects genomic instability and phenotypic evolution in aerobic cells.

Full Text

Duke Authors

Cited Authors

  • Zhang, K; Zheng, D-Q; Sui, Y; Qi, L; Petes, TD

Published Date

  • April 23, 2019

Published In

Volume / Issue

  • 47 / 7

Start / End Page

  • 3521 - 3535

PubMed ID

  • 30668788

Pubmed Central ID

  • 30668788

Electronic International Standard Serial Number (EISSN)

  • 1362-4962

Digital Object Identifier (DOI)

  • 10.1093/nar/gkz027

Language

  • eng

Conference Location

  • England