Skip to main content

Zymogen proteolysis within the pancreatic acinar cell is associated with cellular injury.

Publication ,  Journal Article
Grady, T; Mah'Moud, M; Otani, T; Rhee, S; Lerch, MM; Gorelick, FS
Published in: Am J Physiol
November 1998

The pathological activation of digestive zymogens within the pancreatic acinar cell probably plays a central role in initiating many forms of pancreatitis. To examine the relationship between zymogen activation and acinar cell injury, we investigated the effects of secretagogue treatment on isolated pancreatic acini. Immunofluorescence studies using antibodies to the trypsinogen-activation peptide demonstrated that both CCK (10(-7) M) hyperstimulation and bombesin (10(-5) M) stimulation of isolated acini resulted in trypsinogen processing to trypsin. These treatments also induced the proteolytic processing of procarboxypeptidase A1 to carboxypeptidase A1 (CA1). After CCK hyperstimulation, most CA1 remained in the acinar cell. In contrast, the CA1 generated by bombesin was released from the acinar cell. CCK hyperstimulation of acini was associated with cellular injury, whereas bombesin treatment did not induce injury. These studies suggest that 1) proteolytic zymogen processing occurs within the pancreatic acinar cell and 2) both zymogen activation and the retention of enzymes within the acinar cell may be required to induce injury.

Duke Scholars

Published In

Am J Physiol

DOI

ISSN

0002-9513

Publication Date

November 1998

Volume

275

Issue

5

Start / End Page

G1010 / G1017

Location

United States

Related Subject Headings

  • Trypsinogen
  • Trypsin
  • Rats, Wistar
  • Rats
  • Protein Processing, Post-Translational
  • Pancreas
  • Oligopeptides
  • Male
  • Kinetics
  • Enzyme Precursors
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Grady, T., Mah’Moud, M., Otani, T., Rhee, S., Lerch, M. M., & Gorelick, F. S. (1998). Zymogen proteolysis within the pancreatic acinar cell is associated with cellular injury. Am J Physiol, 275(5), G1010–G1017. https://doi.org/10.1152/ajpgi.1998.275.5.G1010
Grady, T., M. Mah’Moud, T. Otani, S. Rhee, M. M. Lerch, and F. S. Gorelick. “Zymogen proteolysis within the pancreatic acinar cell is associated with cellular injury.Am J Physiol 275, no. 5 (November 1998): G1010–17. https://doi.org/10.1152/ajpgi.1998.275.5.G1010.
Grady T, Mah’Moud M, Otani T, Rhee S, Lerch MM, Gorelick FS. Zymogen proteolysis within the pancreatic acinar cell is associated with cellular injury. Am J Physiol. 1998 Nov;275(5):G1010–7.
Grady, T., et al. “Zymogen proteolysis within the pancreatic acinar cell is associated with cellular injury.Am J Physiol, vol. 275, no. 5, Nov. 1998, pp. G1010–17. Pubmed, doi:10.1152/ajpgi.1998.275.5.G1010.
Grady T, Mah’Moud M, Otani T, Rhee S, Lerch MM, Gorelick FS. Zymogen proteolysis within the pancreatic acinar cell is associated with cellular injury. Am J Physiol. 1998 Nov;275(5):G1010–G1017.

Published In

Am J Physiol

DOI

ISSN

0002-9513

Publication Date

November 1998

Volume

275

Issue

5

Start / End Page

G1010 / G1017

Location

United States

Related Subject Headings

  • Trypsinogen
  • Trypsin
  • Rats, Wistar
  • Rats
  • Protein Processing, Post-Translational
  • Pancreas
  • Oligopeptides
  • Male
  • Kinetics
  • Enzyme Precursors