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Unique and overlapping GLI1 and GLI2 transcriptional targets in neoplastic chondrocytes.

Publication ,  Journal Article
Ali, SA; Niu, B; Cheah, KSE; Alman, B
Published in: PLoS One
2019

Excessive Hedgehog (Hh) signaling in chondrocytes is sufficient to cause formation of enchondroma-like lesions which can progress to chondrosarcoma. To elucidate potential underlying mechanisms, we identified GLI1 and GLI2 target genes in human chondrosarcoma. Using chromatin immunoprecipitation (ChIP) sequencing and microarray data, in silico analyses were conducted to identify and characterize unique and overlapping GLI1 and GLI2 binding regions in neoplastic chondrocytes. After overlaying microarray data from human chondrosarcoma, 204 upregulated and 106 downregulated genes were identified as Hh-responsive Gli binding targets. After overlaying published Gli ChIP-on-chip data from mouse, 48 genes were identified as potential direct downstream targets of Hedgehog signaling with shared GLI binding regions in evolutionarily conserved DNA elements. Among these was BMP2, pointing to potential cross-talk between TGF beta signaling and Hh signaling. Our identification of potential target genes that are unique and common to GLI1 and GLI2 in neoplastic chondrocytes contributes to elucidating potential pathways through which Hh signaling impacts cartilage tumor biology.

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Published In

PLoS One

DOI

EISSN

1932-6203

Publication Date

2019

Volume

14

Issue

1

Start / End Page

e0211333

Location

United States

Related Subject Headings

  • Zinc Finger Protein Gli2
  • Zinc Finger Protein GLI1
  • Tumor Cells, Cultured
  • Signal Transduction
  • Protein Binding
  • Oligonucleotide Array Sequence Analysis
  • Nuclear Proteins
  • Mice
  • Humans
  • Hedgehog Proteins
 

Citation

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Ali, S. A., Niu, B., Cheah, K. S. E., & Alman, B. (2019). Unique and overlapping GLI1 and GLI2 transcriptional targets in neoplastic chondrocytes. PLoS One, 14(1), e0211333. https://doi.org/10.1371/journal.pone.0211333
Ali, Shabana Amanda, Ben Niu, Kathryn S. E. Cheah, and Benjamin Alman. “Unique and overlapping GLI1 and GLI2 transcriptional targets in neoplastic chondrocytes.PLoS One 14, no. 1 (2019): e0211333. https://doi.org/10.1371/journal.pone.0211333.
Ali SA, Niu B, Cheah KSE, Alman B. Unique and overlapping GLI1 and GLI2 transcriptional targets in neoplastic chondrocytes. PLoS One. 2019;14(1):e0211333.
Ali, Shabana Amanda, et al. “Unique and overlapping GLI1 and GLI2 transcriptional targets in neoplastic chondrocytes.PLoS One, vol. 14, no. 1, 2019, p. e0211333. Pubmed, doi:10.1371/journal.pone.0211333.
Ali SA, Niu B, Cheah KSE, Alman B. Unique and overlapping GLI1 and GLI2 transcriptional targets in neoplastic chondrocytes. PLoS One. 2019;14(1):e0211333.

Published In

PLoS One

DOI

EISSN

1932-6203

Publication Date

2019

Volume

14

Issue

1

Start / End Page

e0211333

Location

United States

Related Subject Headings

  • Zinc Finger Protein Gli2
  • Zinc Finger Protein GLI1
  • Tumor Cells, Cultured
  • Signal Transduction
  • Protein Binding
  • Oligonucleotide Array Sequence Analysis
  • Nuclear Proteins
  • Mice
  • Humans
  • Hedgehog Proteins