In vivo RNA editing of point mutations via RNA-guided adenosine deaminases.
Publication
, Journal Article
Katrekar, D; Chen, G; Meluzzi, D; Ganesh, A; Worlikar, A; Shih, Y-R; Varghese, S; Mali, P
Published in: Nat Methods
March 2019
We present in vivo sequence-specific RNA base editing via adenosine deaminases acting on RNA (ADAR) enzymes with associated ADAR guide RNAs (adRNAs). To achieve this, we systematically engineered adRNAs to harness ADARs, and comprehensively evaluated the specificity and activity of the toolsets in vitro and in vivo via two mouse models of human disease. We anticipate that this platform will enable tunable and reversible engineering of cellular RNAs for diverse applications.
Duke Scholars
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Published In
Nat Methods
DOI
EISSN
1548-7105
Publication Date
March 2019
Volume
16
Issue
3
Start / End Page
239 / 242
Location
United States
Related Subject Headings
- RNA, Messenger
- RNA, Guide, CRISPR-Cas Systems
- RNA Splicing
- RNA Editing
- Point Mutation
- Mice
- Humans
- High-Throughput Nucleotide Sequencing
- HEK293 Cells
- Disease Models, Animal
Citation
APA
Chicago
ICMJE
MLA
NLM
Katrekar, D., Chen, G., Meluzzi, D., Ganesh, A., Worlikar, A., Shih, Y.-R., … Mali, P. (2019). In vivo RNA editing of point mutations via RNA-guided adenosine deaminases. Nat Methods, 16(3), 239–242. https://doi.org/10.1038/s41592-019-0323-0
Katrekar, Dhruva, Genghao Chen, Dario Meluzzi, Ashwin Ganesh, Atharv Worlikar, Yu-Ru Shih, Shyni Varghese, and Prashant Mali. “In vivo RNA editing of point mutations via RNA-guided adenosine deaminases.” Nat Methods 16, no. 3 (March 2019): 239–42. https://doi.org/10.1038/s41592-019-0323-0.
Katrekar D, Chen G, Meluzzi D, Ganesh A, Worlikar A, Shih Y-R, et al. In vivo RNA editing of point mutations via RNA-guided adenosine deaminases. Nat Methods. 2019 Mar;16(3):239–42.
Katrekar, Dhruva, et al. “In vivo RNA editing of point mutations via RNA-guided adenosine deaminases.” Nat Methods, vol. 16, no. 3, Mar. 2019, pp. 239–42. Pubmed, doi:10.1038/s41592-019-0323-0.
Katrekar D, Chen G, Meluzzi D, Ganesh A, Worlikar A, Shih Y-R, Varghese S, Mali P. In vivo RNA editing of point mutations via RNA-guided adenosine deaminases. Nat Methods. 2019 Mar;16(3):239–242.
Published In
Nat Methods
DOI
EISSN
1548-7105
Publication Date
March 2019
Volume
16
Issue
3
Start / End Page
239 / 242
Location
United States
Related Subject Headings
- RNA, Messenger
- RNA, Guide, CRISPR-Cas Systems
- RNA Splicing
- RNA Editing
- Point Mutation
- Mice
- Humans
- High-Throughput Nucleotide Sequencing
- HEK293 Cells
- Disease Models, Animal